Colourimetric redox-polyaniline nanoindicator for in situ vesicular trafficking of intracellular transport

Vesicular pH modulates the function of many organelles and plays a pivotal role in cell metabolism processes such as proliferation and apoptosis. Here, we introduce a simple colorimetric redox-polyaniline nanoindicator, which can detect and quantify a broader biogenic pH range with superior sensitivity compared to pre-established trafficking agents employing one-dimensional turn-on of the fluorescence resonance-energy-transfer （FRET） signal. We fabricated polyaniline- based nanoprobes, which exhibited convertible transition states according to the proton levels, as an in situ indicator of vesicular transport pH. Silica-coated Fe304-MnO heterometal nanoparticles were synthesised and utilised as a metal oxidant to polymerise the aniline monomer. Finally, silica-coated polyaniline nanoparticles with adsorbed cyanine dye fluorophores Cy3 and Cy7 （FPSNIcyB and FPSNIcy7） were fabricated as proton-sensitive nanoindicators. Owing to the selective quenching induced by the local pH variations of vesicular transport, FPSNIcy3 and FPSNIcy7 demonstrated excellent intracellular trafficking and provided sensitive optical indication of minute proton levels.

小囊的 pH 调制许多细胞器的功能并且在象增长和 apoptosis 那样的房间新陈代谢过程起一个枢轴的作用。这里，我们介绍简单比色的 redox-polyaniline nanoindicator，它能检测并且确定有与采用荧光 resonance-energy-transfer 的一个维的刺激的预先的 trafficking 代理人相比的优异敏感的一个更宽广的源於生物的 pH 范围(烦恼) 信号。我们制作了基于 polyaniline 的 nanoprobes，它根据质子层次展出了可改变的转变状态，作为一在里面小囊的运输 pH 的 situ 指示物。硅石涂的 Fe < 潜水艇 class= “ a-plus-plus ” > 3 O < 潜水艇 class= “ a-plus-plus ” > 4 -MnO heterometal nanoparticles 是 synthesised 并且作为金属氧化剂利用了到 polymerise 苯胺单体。最后，有吸附的花青染料 fluorophores Cy3 和 Cy7 的硅石涂的 polyaniline nanoparticles (FPSNI < 潜水艇 class= “ a-plus-plus ” > Cy3 和 FPSNI < 潜水艇 class= “ a-plus-plus ” > Cy7 ) 作为质子敏感的 nanoindicators 被制作。由小囊的运输的本地 pH 变化由于选择熄灭导致了， FPSNI < 潜水艇 class= “ a-plus-plus ” > Cy3 和 FPSNI < 潜水艇 class= “ a-plus-plus ” > Cy7 表明了优秀细胞内部的 trafficking 并且提供了极小的质子层次的敏感光指示。