芍药苷预处理对大鼠心肌缺血再灌注损伤的保护作用

Protection against Myocardial Ischemia / Reperfusion Injury in Rats by Pretreatment with Paeoniflorin

目的:观察芍药苷对大鼠心肌缺血再灌注(myocardial ischemia/reperfusion,MI/R)损伤的保护作用,探讨其作用机制。方法:结扎冠脉造成心肌缺血30 min再灌注2 h,建立MI/R损伤大鼠模型,随机分为假手术组、模型组、芍药苷三个剂量组(20,10,5 mg·kg-1)(n=10),于术前1 h和再灌注即刻分别进行尾静脉注射给药。取血清,测定肌酸激酶(CK)、乳酸脱氯酶(LDH)、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量;取心脏,测定心肌梗死面积。结果:芍药苷高、中剂量可明显缩小大鼠心肌梗死面积,与模型组比较差异有统计学意义(P<0.05);芍药苷各剂量组可不同程度降低血清CK和LDH活性(P<0.05或P<0.01),升高SOD活性(P<0.01);芍药苷高、中剂量组降低血清MDA含量,与模型组比较差异有统计学意义(P<0.05)。结论:芍药苷预处理对MI/R损伤的保护作用与其减少自由基的生成、抑制脂质过氧化反应等有关。

Objective： To investigate the protective effects of paeoniflorin on myocardial ischemia/reperfusion （MI/R） injury in rats. Methods： The rat model of MI/R injury was prepared by coronary artery ligation for 30 min followed by 2-hour reperfusion. Then the rats were randomly divided into 5 groups： sham group, model group, paeoniflorin group respectively at high, medium and low dose （20, 10 and 5 ·kg^-1, n = 10）. Paeoniflorin was respectively injected via tail vein 1 h before the operation and at the beginning of the reperfusion. The CK, LDH and SOD activities and MDA contents in serum of rats were examined, and the area of myocardial in- farction was also calculated after the reperfusion. Results： Paeoniflorin （20 and 10 ·kg^-1） could significantly reduce the area of myocardial infarction when compared with the model group （P 〈 0.05 ）. Paeoniflorin could obviously inhibit the CK and LDH activities （P 〈 0.05 or P 〈 0.01 ） and enhance the SOD activity （P 〈 0.01 ） in serum of MI/R rats. The MDA content in paeoniflorin groups at high and medium dose was significantly lower than that in the model group （P 〈 0.05）. Conclusion： Paeoniflorin pretreatment shows effectiveness against MI/R injury, which may be associated with. the inhibition of lipid peroxidation.