柴胡疏肝散对抑郁大鼠5-HT及其羟化酶的影响

Effect of Chaihu Shugan Powder on the content of 5-hydroxytryptamine and the expression of tryptophan hydroxylase 2 in depression rats

目的 观察柴胡疏肝散对抑郁大鼠海马神经递质5-羟色胺（5-HT）含量及中缝核色氨酸羟化酶2（TPH2）表达量的影响.方法 雄性SD大鼠60只,采用随机数字表法分为正常组、利血平模型组、盐酸氟西汀组、柴胡疏肝散低剂组和高剂组,每组12只.小剂量腹腔注射利血平制备抑郁模型大鼠,实验期间对大鼠体质量、行为学改变进行观察,ELISA法测定海马组织单胺类神经递质5-HT含量,Western blot法检测大鼠脑中缝核TPH2表达量.结果 与模型组体质量[（225.02±5.23）g]比较,柴胡疏肝散高剂组体质量[（238.78±5.16）g]增加明显（P＜0.01）;与模型组Open-Field行为学得分[（12.6±5.1）分]比较,柴胡疏肝散高剂组可以改善抑郁大鼠行为[（15.6±7.8）分,P＜0.05].与模型组大鼠脑内5-HT含量[（1.09±0.27）ng/ml]和TPH2表达量[（0.16±0.04）]比较,高剂量柴胡疏肝散对两者的提高十分明显[分别为（1.80±0.58） ng/ml,（0.66±0.21）],差异有统计学意义（P＜0.01）.结论 中药柴胡疏肝散抗抑郁效果显著,可能与增加大鼠海马组织5-HT含量、提高脑中缝核TPH2表达量有关.

Objective To explore the effect of Chaihu Shugan Powder （CSP） on the content of 5-hydroxytryptamine （5-HT） in the hippocampus and the expression of tryptophan hydroxylase 2（TPH2） in median raphe nuclei in depression rats.Methods SD rats were randomly divided into normal group（n=12）,model group （n=12）,fluoxetine group （n=12）,low-dose and high-dose CSP group （n =12,respectively）.Depression model was made by reserpine intraperitoneal injection.During the experiment,the weight and the open-field scores were calculated;the content of 5-HT was detected by ELISA.The expression of TPH2 in median raphe nuclei was detected by Western blot.Results Compared with the weight （（225.02±5.23） g）,the open-field scores （（12.6± 5.1）score） and content of 5-HT （（1.09±0.27） ng/ml） in the model group,high-dose CSP showed significantly improve the depressive rats in weight,open field score and content of 5-HT （（238.78±5.16） g,（15.6±7.8） score and （1.80±0.58） ng/ml,respectively;P＜0.05 or P＜0.01）.The expression of TPH2 （0.66±0.21） in median raphe nuclei in the high-dose CSP group was apparently increased compared with that in the model group（0.16±0.04） （P ＜0.01）.Conclusion CSP have the effects of anti-depression,which could be related with the increase of the 5-HT content in the hippocampus and the expression of TPH2 in median raphe nuclei.