多氯联苯118引起大鼠甲状腺炎症反应的研究

A study of polychlorinated biphenyl 118 induced inflammation reaction in rat thyroid

目的 ：探讨持续低浓度多氯联苯118（polychlorinated biphenyl 118,PCB118）暴露是否会引起大鼠甲状腺炎症反应。方法：1将40只清洁级成年雄性Wistar大鼠[体重（200±10）g]随机分为4组,每组10只,分别给予溶剂对照玉米油及低、中、高剂量[10、100、1 000μg/（kg·d）]的PCB118,每周腹腔注射5 d,每周称重,造模13周后取甲状腺组织进行HE染色,观察炎症细胞浸润情况;2CCK8实验用于检测PCB118对细胞活力的影响;3实时定量PCR（q RT-PCR）实验检测PCB118对炎症因子白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）及细胞间黏附分子-1（ICAM-1）m RNA表达水平的影响。结果：1大鼠甲状腺组织HE染色可见各PCB118处理组有大量炎症细胞浸润;2相对低浓度的PCB118（0-25 nmol/L）对细胞活力无显著影响（P〉0.05）;3与对照组比较,不同低浓度的PCB118刺激后,IL-6、TNF-α及ICAM-1的m RNA表达量显著增加（P〈0.05）。结论 ：PCB118可引起大鼠甲状腺组织及甲状腺细胞发生炎症反应。

Objective：To explore whether polychlorinated biphenyl ll8（PCBll8）could cause inflammation reaction in rat thyroid. Methods： （!）Forty male Wistar rats were randomly divided into 4 groups：solvent control group（corn oil）,low dose, medium dose and high dose PCB 118 groups, intraperitoneal injected with corn oil or different doses of PC B 118（ 10,100,1 000 μg/（kg. d）, respectively） for 5 d per week, and weekly weighed. Thirteen weeks later, the thyroid tissues were stained using HE method and the infiltration of inflammatory cells were observed. （2） The effects of PCB118 on FRTL-5 cells viability was assessed using a Cell Counting Kit-8 assay. （3）Quantitative real-time PCR（qRT-PCR）was used to detect the mRNA expression of cytokines interleukin-6（IL-6）,tumor necrosis factor-a （TNF-a） and intercellular adhesion molecule-l（ICAM-1 ）. Results： （4）In HE staining,there were a large number of inflammatory cells infiltration in PCB118-treated rat thyroid tissue; （2）Relatively low concentrations of PCB118 had no significant effects on cells viability （P 〉 0.05）; （3）Compared to the control group,the mRNA levels of IL-6,TNF-a and ICAM-I increased significantly （P 〈 0.05） after stimulating with different concentrations of PCB118. Conclusion： PCB118 could induce inflammation reaction in rat thyroid tissues.