长链非编码RNA H19基因多态性与早发冠心病患者易感性研究

Association between polymorphisms of long non-coding RNA H19 and susceptibility to premature coronary artery disease

目的 ：探讨编码一段长链非编码RNA的H19基因多态性与早发冠心病（p CAD）患者易感性的相关关系。方法 ：采用Taq Man技术分析213例早发冠心病患者和776例对照的H19基因4个多态位点（rs2067051、rs2251375、rs217727、rs4929984）的基因型,用SPSS软件进行统计学分析。结果 ：早发冠心病组H19基因rs217727位点的CT基因型、TT基因型及T等位基因分布频率均高于对照组。多元Logistic回归分析显示：rs217727多态性与早发冠心病发病独立相关（P〈0.01）。T等位基因携带者、TT纯合基因患早发冠心病的风险分别是CC纯合基因型的2.42倍（OR=2.42,95%CI=1.55-3.71）和3.01倍（OR=3.01,95%CI=1.87-4.85）。结论：H19 rs217727多态性与早发冠心病易感性有关,T等位基因可能是早发冠心病的遗传易感因素。

Objective：To study the association between polymorphisms in H19 gene,which transcribes a long non-coding RNA, and susceptibility to premature coronary artery disease （pCAD）. Methods： Four polymorphisms （rs2067051,rs2251375,rs217727, rs4929984） of H19 gene were analyzed in 213 pCAD patients and 776 control subjects. Polymorphisms were genotyped by TaqMan technology. The statistical analysis was implemented in SPSS. Results： The frequencies of genotype CT,Tr and allele T in pCAD group of rs217727 were higher than that in the control group. Multivariate logistic regression analysis showed that the rs217727 polymorhpism of H19 gene was independently associated with the occurrence of pCAD（P 〈 0.01）. The risk of patients with T allele and TIhomozygous genotype was 2.42 times（OR=2.42,95%CI=1.56-3.71）and 3.01 times（OR=3.01,95%CI=l.87-4.85）than that of patients with CC homozygous genotype. Conclusion： H19 gene rs217727 polymorphism is associated with the susceptibility of pCAD, and T allele may be a genetic susceptibility factor of pCAD.