阻塞性睡眠呼吸暂停低通气综合征血浆尾加压素Ⅱ检测及其意义

Obstructive Sleep Apnea Hypopnea Syndrome of Plasma Urotensin Ⅱ Detection and Its Significance

目的阻塞性睡眠呼吸暂停低通气综合征(OSAHS)大鼠模型组织和血浆中UⅡ及其受体GPR14的基因表达和蛋白含量进行研究,阐明OSAHS时水平的变化,分析其在OSAHS发生发展中的作用。方法采集OSAHS模型大鼠组织标本和血样以及患者血样,采用RTPCR方法对大鼠组织内UⅡ及GPR14 m RNA表达进行检测。结果模型组大鼠的氧饱和度、血浆UⅡ含量、气管组织中UⅡ及GPR14的含量与正常组相比(P<0.05);模型组大鼠气管组织内UⅡ和GPR14的m RNA的表达(P<0.05)。结论 OSAHS时,SpO2与UⅡ含量呈现反变关系,血浆中UⅡ含量升高,气管组织内UⅡ及GPR14含量及m RNA表达增强,加速OSAHS的发生发展过程。

Objective Research on gene expression and protein contenta of UII and its receptor GPR14 in tissue and plasma of OSAHS rat model,to describe the changing levels of protein content,and analysis of its role in the development of OSAHS.Methods Colect tissue samples and blood samples of OSAHS rat model and blood samples of patients. Use RT-PCR method to detect UII and GPR14 mRNA expression in rat tissues（P〈 0.05）. ResultsOxygen saturation ,content of plasma UⅡ,UⅡ in trachea tissueand,content of GPR14 and the expression of UⅡ rat tracheal tissue the mRNA of GPR14 in the OSAHS group are statistical significance that comparing with the normal group.Conclusion When OSAHS,SpO2 and UⅡ content showed inversed relationship.UⅡ plasma levels increased,intratracheal organization UⅡ and GPR14 levels and mRNA expression increased, accelerating the development of OSAHS.