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人骨肉瘤细胞中sFlt-1转基因表达的测定

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摘要 目的评估sFlt-1基因针对人骨肉瘤G-292细胞体外转导的效率。方法逆转录病毒介导的sFlt-1基因体外转染人骨肉瘤G-292细胞,应用ELISA试验评估sFlt-1转基因表达的效率。结果 sFlt-1转导后3 d检测到了高水平表达,21 d时达到峰值;转基因产物较高水平的表达至少维持了8周。结论 ELISA试验测定证实sFlt-1基因体外转导成功。
出处 《中国医学工程》 2015年第3期43-43,45,共2页 China Medical Engineering
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