三氯乙烯药疹样皮炎患者细胞因子水平分析

Serum cytokines levels in patients with trichloroethylene induced medicamentosa-like dermatitis

目的探讨三氯乙烯（TCE）药疹样皮炎患者血清γ干扰素（IFN-γ）、肿瘤坏死因子（TNF-α）、白细胞介素4（IL-4）和白细胞介素10（IL-10）细胞因子水平的变化。方法采用酶联免疫吸附法（ELISA）测定52例患者和52例正常人血清中IFN-γ、TNF-α、IL-4、IL-10水平,并比较其水平差异,同时分析患者各细胞因子水平的相关性。结果病例组血清中IFN-γ、TNF-α、IL-4、IL-10水平[中位值（范围）]分别为20.59（12.64-198.00）、53.46（26.14-306.60）、28.05（12.83-256.36）、12.77（4.48-245.05）ng/L,对照组分别为13.62（6.03-28.18）、19.97（11.72-43.81）、12.34（10.39-22.39）、5.63（2.77-18.77）ng/L,以上4种结果在两组间比较,差异均有统计学意义（z=-5.649、-5.891、-5.416、-5.264,P〈0.01）;病例组血清中IFN-γ、TNF-α、IL-4、IL-10四种细胞因子水平均相互呈正相关（P〈0.01）。结论 IFN-γ、TNF-α、IL-4、IL-10在TCE药疹样皮炎发生过程中具有重要意义,可用于预测工人发病倾向以及发病趋势。

Objective To observe the level changes of serum cytokines including interferon（IFN-γ）,tumor necrosis factor（TNF-α）,interleukin 4（IL-4） and interleukin 10（IL-10）in patients with trichloroethylene induced medicamentosa-like dermatitis（DMLT）,to help understand the toxicity mechanism. Methods ELISA was used to detect serum IFN-γ,TNF-α,IL-4 and IL-10 concentrations in 52 DMLT patients and 52 controls. Results The levels of Serum IFN-γ,TNF-α,IL-4 and IL-10 were 20.59（12.64- 198.00）,53.46（26.14- 306.60）,28.05（12.83- 256.36）,12.77（4.48- 245.05） ng /L in the DMLT patients,while were 13.62（6.03- 28.18）,19.97（11.72- 43.81）,12.34（10.39- 22.39）,5.63（2.77- 18.77）ng /L in the control group,respectively. The serum levels of IFN-γ,TNF-α,IL-4 and IL-10 in the case group were higher than those in the control group（z =-5.649,-5.891,-5.416,-5.264,respectively,P〈0.01）.There were positive correlations among these cytokines levels.Conclusion IFN-γ,TNF-α,IL-4 and IL-10 maybe play important roles in the pathogenesis of DMLT.