手足口病患儿甘露醇结合凝集素基因多态性与其蛋白水平的研究

Study of MBL gene polymorphism and MBL protein level of children with hand-foot-and-mouth disease(HFMD)

目的研究不同病情的手足口病(HFMD)患儿甘露醇结合凝集素(MBL)的基因分型及其蛋白表达水平,探析MBL与HFMD的相关性。方法选择2012年7月-2014年7月收治的200例HFMD患儿,根据不同病情分为3组,一般病例组68例、重症病例组66例、危重病例组66例,采用序列分析法检测所有患儿MBL基因及分型,酶联免疫吸附试验(ELISA)测定血浆MBL蛋白水平并进行比较分析。结果 200例患儿标本中检测到肠道病毒132株,其中肠道病毒71型(EV71)96株占72.7%、柯萨奇病毒14株占10.6%,3组患儿均以EV71感染为主,但一般病例组EV71检出率均显著低于重症及危重症组,而重症及危重症组均未检测出CoxA16,差异均有统计学意义(P<0.05);一般病例组HYPA基因检出频率显著高于重症、危重组,重症组显著高于危重组,一般病例组LXPA基因检出频率显著低于重症、危重组,重症组显著低于危重组;一般病例组患儿MBL蛋白水平(624.8±114.6)ng/ml,显著高于重症及危重症组,重症组患儿MBL蛋白水平(542.7±109.4)ng/ml显著高于危重症组(468.6±94.9)ng/ml,差异均有统计学意义(P<0.05)。结论 MBL基因多态性所致MBL蛋白水平下降与手足口病患儿的病情具有相关性,MBL对手足口病患儿感染具有保护作用。

OBJECTIVE To study MBL gene polymorphism and MBL protein level of children with different state of hand‐foot‐and‐mouth disease so as to discuss the relevance of MBL and HFMD .METHODS Totally 200 children with HFMD in our hospital during Jul .1021 to Jul .2014 were chosen and were divided into 3 groups according to different conditions of diseases with 68 cases in ordinary case group ,66 cases in severe case group and 66 cases in critical case group .MBL gene and types of all children were detected by series analysis method and plasma MBL protein level was detected by ELISA .The results of the 2 groups were compared and analyzed .RESULTS A total of 132 strains of enterovirus were detected from the 200 samples , including 96 strains of EV71 enterovirus （72 .7% ） and 14 strains of Coxsackie virus （10 .6% ） .EV71 was the major pathogens infected by three groups of children .However ,the detection rate of EV71 in ordinary case group was obviously higher than severe case group and critical case group and the latter two groups were not detected with CoxA 16 .The differences were significant （P〈0 .05） .HYPA gene frequency of ordinary case group was obviously higher than severe case group and critical case group ,and severe case group was obviously higher than critical group .LXPA gene frequency of ordinary case group was obviously lower than severe case group and critical case group ,and severe case group was obviously lower than critical group .MBL plasma level of ordinary case group was （624 .8 ± 114 .6） ng/ml ,which was obvi‐ously higher than severe case group and critical case group ,and MBL plasma level of severe case group was （542 .7 ± 109 .4） ng/ml and was obviously higher than critical group of （468 .6 ± 94 .9） ng/ml （P〈0 .05） .CONCLUSION MBL plasma level drop caused by MBL gene polymorphism has relevance with condition of disease of children with HFMD .MBL plays a role of protection for infection of children with HFMD .