HPLC法同时测定三维葡磷钙咀嚼片中维生素B_1、维生素B_2的含量

Content Determination of Vitamin B_1 and Vitamin B_2 in Trivitamin and Calcium Gluconate,Calcium Hydrogen Phosphate Chewable Tablets by HPLC

目的：建立同时测定三维葡磷钙咀嚼片中维生素B1、维生素B2含量的方法。方法：采用高效液相色谱法。色谱柱为Inertsil C18,流动相为含1%三乙胺的0.02 mol/L庚烷磺酸钠溶液（磷酸调p H至5.5）-甲醇（74∶26,V/V）,检测波长为260 nm,流速为1.0 ml/min,柱温为30℃,进样量为20μl。结果：维生素B1、维生素B2质量浓度分别在0.22~1.10、0.22~1.09μg/ml范围内与各自峰面积呈良好的线性关系（r=0.999 8、0.999 7）;精密度、稳定性、重复性试验的RSD〈1%;平均加样回收率分别为99.66%、100.31%,RSD分别为0.96%、0.92%（n=9）。结论：本方法简便、准确度高,可用于三维葡磷钙咀嚼片中维生素B1、维生素B2的含量测定。

OBJECTIVE：To establish a method for the content determination of vitamin B1 and vitamin B2 in Trivitamin and calcium gluconate,calcium hydrogen phosphate chewable tablets. METHODS：HPLC was conducted. The column was Inertsil C18 with the mobile phase of 1% triethylamine contained 0.02 mol/L of sodium heptane solution（p H was adjusted to 5.5 by phosphate）- methanol（74 ∶ 26,V/V）at the flow rate of 1.0 ml/min,the detection wavelength was 260 nm,temperature was 30 ℃ and the volume was 20 μl.RESULTS：There was a good linear relationship between the quality concentration of vitamin B1 and peak area in the range of 0.22-1.10μg/ml（r=0.999 8）and vitamin B2 was in the range of 0.22-1.09 μg/ml（r=0.999 7）. The RSDs of precision,reproducibility and stability tests were all less than 1%;the average recovery was respectively 99.66%（RSD=0.96%,n=9）and 100.31%（RSD=0.92%,n=9）. CONCLUSIONS：The method is simple,accurate,sensitive and reliable and suitable for the content determination of vitamin B1 and vitamin B2 in Trivitamin and calcium gluconate,calcium hydrogen phosphate chewable tablets.