2型糖尿病患者循环内皮微颗粒的变化与内皮一氧化氮合酶的关系

Change of endothelial microparticles in patients with type 2 diabetes and the association with the ndothelial nitric oxide synthase

目的探讨2型糖尿病（T2DM）患者循环内皮微颗粒（EMPs）水平与内皮一氧化氮合酶（eNOS）的关系。方法采用流式细胞仪技术测定105例糖尿病患者和40例健康对照者血清EMPs水平，ELISA法检测eNOS水平，分析EMPs与eNOS的关系。结果①与健康对照组相比，T2DM组循环EMPs水平显著增高，eNOS水平明显下降；②循环EMPs与eNOS呈负相关。③以eNOS为因变量的多因素回归分析显示，循环内皮微颗粒水平是其独立影响因素。结论T2DM患者循环EMPs增加，eNOS水平下降，EMPs水平是eNOS水平的独立影响因素，提示12DM患者体内较高的EMPs水平加速了大动脉弹性功能减退。

Objective To investigate the association of endothelial microparticles （EMPs） and endothelial nitric oxide synthase（eNOS）. Methods The level of circulating EMPs was measured by flow cytometer and the level of eNOS was measured by ELISA in healthy volunteers （n = 40） and patients with T2DM （n = 105 ）. Results Compared with the control group,the level of circulating EMPs was increased in T2DM, but the level of eNOS was decreased. There was a close correlation between the level of circulating EMPs and the level of eNOS. Conclusions There are elevated circulating EMPs and impaired arterial elasticity in patients with T2DM, The level of circulating EMPs correlates with eNOS, which suggestes high level of circulating EMPs may impair arterial elasticity in patients with T2DM.