空气中丙烯酰胺的高效液相色谱法研究

Study on the high performance liquid chromatography(HPLC)method of acrylamide in air

目的通过实验,建立了高效液相色谱法测定工作场所空气中丙烯酰胺含量的测定方法。方法样品经吸收液采集后,用高效液相色谱分析,外标法定量。本法采用ODS-3反相色谱柱(4.6 mm×150 mm),波长为200 nm,流动相为甲醇-水(5∶95,V/V),流速为1.000 ml/min,进行等梯度洗脱,保留时间为3.376 min。结果丙烯酰胺的浓度为0.0μg/ml^2.0μg/ml时,线性关系良好,线性方程为y=4.425×10-6x-0.008,相关系数(r)为1.0。本法的检出限为9.9×10-5μg/ml,加标回收率为98.1%~100.0%,RSD为0.08%~0.30%。结论本法样品无需处理,直接进样,出峰快、方便快捷、灵敏度高、线性范围宽、重复性好、定性定量准确,适合工作场所空气中丙烯酰胺的测定。

Objective Through the experiment, to establish a method for determination the acrlamide in the working place air by a high performance liquid chromatography method. Methods Samples were collected by absorbing liquid, and analyzed by high performance liquid chromatography （ HPLC）, and quantified by external standard method. This method adopted the ODS - 3 reversed phase chromatographic column （4.6 mm × 150 mm）, wavelength of 200 nm, mobile phase of methanol - water （5 ： 95, V/V）, the flow rate of 1. 000 ml/min to conduct gradient elution, and the retention time was 3. 376 min. Results When the concentration of acrylamide was of 0.0 μg/ml - 2.0 μg/ml, the linear relationship was good, the linear equation was y = 4. 425 × 10^-6x -0. 008, and the related coefficient （r） was 1.0. The detection limit was 9.9 × 10^-5 μg/ml, the standard addition recovery rate was 98.1% - 100.0%, and the relative deviation was 0.08% -0.30%. Conclusion In this method, the samples needn＇t deal with, and went in directly; the peak appeared fast. This method is convenient and quick, with high sensitivity, good wide linear range, good repeatability, qualitative accuracyand quantitative accuracy. It is suitable for the deter- mination of aerylamide in workplace air.