胶东半岛地区鸡源空肠弯曲杆菌毒力基因携带状况调查

Analysis of virulence gene of Campylobacter jejuni Isolates in Jiaodong peninsula

为了了解胶东半岛地区鸡源空肠弯曲杆菌的毒力基因携带和流行情况,对胶东半岛不同来源的样品进行本菌的分离鉴定,用PCR法对分离菌株的14种毒力基因进行了检测。结果:胶东半岛地区鸡源空肠弯曲杆菌的平均分离率为45%。14种检测的毒力因子cia B、rac R、dna K、pld A、cdt A、cdt B、cdt C、neu B1、che W、grp E、che Y、fla A、peb1A cad F的检出率分别为96.26%、97.2%、100%、100%、76.64%、99.07%、99.07%、4.67%、99.07%、71.96%、98.13%、100%、99.07%和99.07%。结果表明,胶东半岛地区鸡源空肠弯曲杆菌毒力基因携带率高,不同毒力基因携带率差异大。所以,加强空肠弯曲杆菌毒力基因的监测,对公共卫生安全意义重大。

To investigate the prevalence of virulence genes of Campylobacter jejuni from chicken in Jiaodong peninsula, we isolated and identified Campylobacter jejuni from 238 samples, then detected 14 virulence genes in the isolates. The result showed that the separation rate of Campylobacter jejuni was 45%, the detection rate of the genes ciaB, racR, dnaK,pldA, cdtA, cdtB, cdtC, neuB1, cheW, grpE, cheY ,flaA ,peblA cadF were 96.26%, 97.2%, 100%, 100%, 76.64%, 99.07%, 99.07%, 4.67%, 99.07%, 71.96%, 98.13%, 100%, 99.07% and 99.07%. Campylobacter jejuni separated from Jiaodong peninsula carried manifold virulence genes which distributed differently. Therefore, strengthening the detection of Campylobacter jejuni and its virulence genes is significant to public health security.