紫杉醇对Caski细胞增殖及APC、DKK-3基因表达的影响

Effects of Paclitaxel on Cell Proliferation and Expression of APC and DKK-3 in Human Cervical Cancer Caski Cells

目的研究紫杉醇对Caski细胞增殖及APC、DKK-3基因表达的影响。方法用MTT法、RT-PCR法及甲基化特异性PCR分别检测紫杉醇对人宫颈癌Caski细胞株增殖的影响、APC与DKK-3 mRNA表达水平、APC与DKK-3启动子甲基化水平。结果≥1μmol/L紫杉醇能明显抑制人宫颈癌Caski细胞株的增殖(P<0.05);从5μmol/L开始,随着紫杉醇给药剂量的增大,Caski细胞APC、DKK-3 mRNA表达水平明显增加(P<0.05),而Caski细胞APC、DKK-3启动子甲基化水平显著降低(P<0.05)。结论紫杉醇能呈剂量依赖性的抑制Caski细胞的增殖并上调APC、DKK-3 mRNA的表达,这可能与其诱导APC、DKK-3启动子的去甲基化有关。

Objective To study the effect of paclitaxel on the proliferation of Caski cells and the expression of APC, DKK-3 gene. Methods MTT colorimetric method, RT-PCR method and methylation specific PCR were used to detect the proliferation effects, the mRNA expression level of APC and DKK-3, APC and DKK-3 promoter methylation level induced by paclitaxel on human cervical carcinoma Caski cell line,respectively. Results Paclitaxel significantly inhibited the prolif- eration of Caski cervical cancer cell line when the concentration of Paclitaxel was more than I i.Lmol/L （ P 〈 0. 05） ; Starting from concentration of 5 Ixmol/L, with increasing of paclitaxel dosage, APC and DKK-3 mRNA expression level increased sig- nificantly （ P 〈 0.05）, while APC and DKK-3 promoter methylation level significantly decreased （ P 〈 0. 05 ）. Conclu- tion Paclitaxel could dose-dependently inhibit the proliferation of Caski cells through increasing APC and DKK-3 mRNA expression, which may be related with the inhibition of APC and DKK-3 promoter methylation.