血管内皮生长因子和低氧诱导因子1α在颈动脉粥样硬化性狭窄患者血清中的表达及临床意义

Expression and Clinical Significances of Serum VEGF and HIF-1α in Patients with Carotid Artery Stenosis

目的探讨颈动脉粥样硬化性狭窄患者血清中血管内皮生长因子(VEGF)、低氧诱导因子1α(HIF-1α)的变化及临床意义。方法采用酶连免疫吸附反应(ELISA)方法检测92例颈动脉粥样硬化性狭窄(CAS)患者和30例健康查体者血清中VEGF、HIF-1α水平。将92例患者根据颈动脉粥样硬化性狭窄程度分为轻度狭窄、中度狭窄、重度狭窄、极重度狭窄,记录患者一般临床资料,并分析CAS发生的危险因素。结果 CAS组高血压、糖尿病发生率显著高于对照组(P<0.05),随颈动脉狭窄程度的加重SBP和FPG水平逐渐升高。CAS组VEGF、HIF-1α均显著高于对照组,相比较有显著性差异(t=6.807、7.500,P<0.01)。CAS患者按照颈动脉粥样硬化性狭窄标准进行分级后发现血清VEGF、HIF-1α水平随着颈动脉狭窄程度的加重而逐渐上升,CAS患者的VEGF与HIF-1α水平呈正相关性。结论VEGF和HIF-1α在颈动脉粥样硬化性狭窄患者血清中呈高表达,其表达水平随着颈动脉狭窄程度的加重而升高,并且血清VEGF水平随着HIF-1α的变化而改变。血清VEGF和HIF-1α高水平可能成为CAS发生和发展的危险因素。

Objective To study the expression and clinical significance of serum vascular endothelial growth factor （VEGF）and hypoxia inducible factor 1 α （HIF-1α ）in patients with carotid artery stenosis （CAS）. Methods The serum VEGF and HIF-loLlevels from 92 cases of patients with CAS and 30 cases nor- mal person（control group）were detected by ELISA method. 92 cases of patients with CAS were divided into mild stenosis group, moderate stenosis group, severe stenosis group and very severe stenosis group according to the degree of carotid artery stenosis, the clinical data were recorded and the risk factors of CAS were analyzed. Results The hypertension and diabetes incidence of CAS group were significantly higher than the normal control group （P 〈 0.05 ） , the SBP and FPG levels gradually increased with the severity of stenosis. The VEGF and HIF- 1α levels of CAS group were significantly higher than the control group, the difference was significant （t = 6. 807 and 7. 500,P 〈0.01 ）. According to the grouping of degree of carotid artery stenosis, this study found that the VEGF and HIF-1 α levels increased with carotid artery stenosis aggravated. There was a positive correlation between VEGF level and HIF-1 α devel of CAS group. Conclusion The serum VEGF and HIF- 1 α levels have high expression in patients with CAS, the expression levels of VEGF and HIF- 1 α increased with carotid artery stenosis aggravated, and the VEGF levels changes with the change of HIF-1 α levels. The high level of＇ VEGF and HIF-1 α may be risk factors for the occurrence and incidence of the CAS.