大黄对脓毒症大鼠肠黏膜损伤的影响：与乌司他丁比较

Effect of rhubarb on intestinal mucosal damage in septic rats： a comparison with ulinastatin

目的通过与乌司他丁比较，评价大黄对脓毒症大鼠肠黏膜损伤的影响。方法健康SD大鼠84只，3月龄，体重200～330g，雌雄各半，采用随机数字表法，将其分为5组：对照组（c组，n=6）、假手术组（s组，n=6）、脓毒症组（Sep组，n=24）、大黄组（R组，n=24）和乌司他丁组（u组，n=24）。采用盲肠结扎穿孔的方法制备大鼠脓毒症模型。R组大黄粉1．2g／100g溶于室温生理盐水中泡制3—4h后取全部滤液，约2～3m1，灌胃1次／12h，72h后制备脓毒症模型；U组腹腔注射乌司他丁50000U／kg，加生理盐水至2ml，1次／24h，72h后制备脓毒症模型。Sep组、R组和u组于结扎盲肠后6、12、24和48h（T。）时采集眼眶静脉丛血样，采用ELSIA法测定血浆二胺氧化酶（DAO）活性。结果与C组和S组比较，Sep组、R组和U组T1-4时血浆DAO活性升高（P〈0．05）；与Sep组比较，R组和U组T3,4时血浆DAO活性降低（P〈0．05）；R组和U组T1-4时血浆DAO活性差异无统计学意义（P〉0．05）。结论大黄减轻脓毒症大鼠肠黏膜损伤的效应与乌司他丁相似。

Objective To evaluate the effect of rhubarb on intestinal mucosal damage in septic rats by comparing with ulinastatin. Methods A total of 84 healthy Sprague-Dawley rats （half male, half fe- male） , aged 3 months, weighing 200-330 g, were randomly divided into 5 groups according to the ran- dom number table： control group （group C, n = 6） , sham operation group （group S, n = 6） , sepsis group （group Sep）, rhubarb group （group R, n= 24） and ulinastatin group （group U, n= 24）. Sepsis was induced by cecum ligation and puncture. In group R, rhubarb 1.2 g/100 g was dissolved in normal sa- line at room temperature, 3 and 4 h later the filtrate about 2-3 ml was obtained and injected through a gas- tric tube into stomach once every 12 h, and 72 h later sepsis was induced. In group U, ulinastatin 50 000 U/kg （in 2 ml of normal saline） was injected once every 24 h, and 72 h later sepsis was induced. In Sep, R and U groups, at 6, 12, 24 and 48 h after ligation （T1-4） , blood samples were collected from the orbit- al venous plexus for determination of plasma diamine oxidase （DAO） activity. Results The activity of plasma DAO was significantly higher at T1-4 in Sep, R and U groups than in C and S groups. The activity of plasma DAO was significantly lower at T3-4 in R and U groups than in Sep group. There was no statistical difference in the plasma DAO activity between R group and U group. Conclusion Rhubarb can reduce in- testinal mucosal damage in septic rats, which is similar to that of ulinastatin.