参芎注射液对缺血再灌注损伤大鼠炎症损伤的影响

Effect of Shenxiong injection on inflammation injury of ischemiareperfusion injury rats

为研究参芎注射液对脑缺血再灌注老龄大鼠炎症损伤的影响。将84只SD大鼠随机分为假手术组、模型组、尼莫通组、参芎注射液高、中、低剂量组。连续腹腔注射给药3 d,采用线栓法建立大鼠脑缺血再灌注模型,再灌注24 h,神经功能评分,取脑TTC染色,ELISA法检测血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)的含量,荧光实时定量PCR(RTPCR)对海马区IL-1β,TNF-α,ICAM-1和MMP-9 m RNA的表达量进行检测。结果,参芎注射液可使脑梗死体积减少,明显改善神经功能,参芎注射液能减少IL-1β,TNF-α,ICAM-1和MMP-9 m RNA的表达量,并减少IL-1β和TNF-α的含量。表明参芎注射液对大鼠缺血再灌注损伤具有明显的保护作用,其机制可能与抑制炎症因子表达有关。

To investigate the effect of Shenxiong injection on the inflammation injury of ischemia-reperfusion injury senile rats. Totally 84 Sprague-Dawley （SD） rats were randomly divided into six groups： the sham operation group, the model group, the Nimodipine group and the Shenxiong injection（low, middle, and high dosage） groups. The rat cerebral ischemia-reperfusion model was established through intraperitoneal injection for 3 d and middle cerebral artery occlusion （MCAO）. After the reperfusion for 24 h, efforts were made to give neurological score, collect brains for TTC staining, detect tumor necrosis factor-α（TNF-α） and interleukin-1β（IL-1β） content in serum by enzyme-linked immunosorbent assay （ELISA） method and measure IL-1β, ICAM-1 and MMP-9 mRNA expressions in hippocampal area by Real-time PCR （RT-PCR）. According to the results, Shenxiong injection could decrease the cerebra[ infarction volume, greatly improved the neurological function and reduce IL-1β, TNF-α, ICAM-1 and MMP-9 mRNA expressions and IL-1β and TNF-α contents. In conclusion, Shenxiong injection shows the significant protective effect on ischemia-reperfusion injury in rats. Its mechanism may be related to the inhibition of inflammatory factor expression.