黔产苗药“薄丈达”中不同极性组分DPPH自由基清除活性的研究

Study on scavenging activity to DPPH free radical of different polarity components in Guizhou Miao medicine “bod zangd dak”

为进一步开发贵州药用植物新资源,寻找更多天然抗氧化剂,该实验以黔产苗药"薄丈达"(bod zangd dak)为原料,利用60%乙醇等为溶剂,通过提取分离得到该药材中不同极性组分,以抗坏血酸为阳性对照,采用DPPH自由基(1,1-二苯基-2-苦肼基自由基)清除法评价苗药"薄丈达"中不同极性组分的抗氧化活性。结果黔产苗药"薄丈达"中不同极性组分的IC50依次为抗坏血酸(0.033 4 g·L-1)<乙酸乙酯部位(0.052 3 g·L-1)<总鞣质部位(0.054 9 g·L-1)<60%乙醇提取物部位(0.076 7 g·L-1)<正丁醇部位(0.110 g·L-1)<水溶性多糖部位(0.168 g·L-1)<水提取物部位(0.174 g·L-1)<萃取后水部位(0.226 g·L-1)<总多糖部位(0.645 g·L-1)。研究表明黔产苗药"薄丈达"中不同极性组分均有不同程度的清除DPPH自由基活性,这为进一步寻找其活性成分和研究抗氧化活性机制提供一定的实验依据。

The paper is aimed to search more natural plant antioxidants and further research and develop new medicinal plant resources in Guizhou. The Guizhou special miao medicine ＂bod zangd dak＂ was extracted with 60% ethanol. The antioxidant activity of the different polarity components separated from the extract was tested by DPPH method with ascorbic acid as positive control. The results showed that the IC50 of the different polarity components was as following： ascorbic acid （0.033 4 g·L^-1） 〈 ethyl acetate components （0. 052 3 g ·L^-1 ） 〈total tannins components （0.054 9 g ·L^-1 ） 〈 60% ethanol extraction components（0. 076 7 g ·L^-1 ） 〈 butanol extraction components （0.110 g·L^-1 ） 〈 water-soluble polysaccharides components （0.168 g·L^-1 ） 〈 water extraction components （0. 174 g ·L^-1 ） 〈 water components after extraction （0.226 g·L^-1 ） 〈 total polysaccharides components（0.645 g·L^-1 ）. It is concluded that the different polarity components have different free radical scavenging activity and that provides a scientific basis for further search of the active ingredients and the activive mechanism.