慢性氯化钴处理对Caspase-3凋亡基因在糖尿病大鼠心肌梗死区表达的影响

Influence of chronic cobalt chloride treatment on expression of Caspase-3 apoptosis gene within myocardial infarction area in GK diabetic rats

目的：探讨慢性氯化钴处理对GK糖尿病大鼠血糖及心肌细胞凋亡的影响。方法：应用体重200-250g的雄性GK糖尿病大鼠及其对照雄性Wistar大鼠,制作大鼠在体心梗模型,制作前测量血糖,手术成功后大鼠被分为六组：Wistar假手术组、Wistar心梗组、Wistar心梗＋氯化钴组、GK假手术组、GK心梗组和GK心梗＋氯化钴组,每组至少存活6只。3周后,测量血糖,剪取心脏,检测缺血坏死区Caspase-3凋亡基因的表达。结果：（1）术后3周,与GK假手术组及心梗组相比,GK心梗＋氯化钴处理组血糖降低[（27.73±2.58）mmol/L、（27.87±3.18）mmol/L比（16.3±2.15）mmol/L],P均〈0.05;（2）与GK心梗组相比,GK心梗＋氯化钴处理组Caspase-3光密度值[（0.84±0.03）比（0.70±0.03）]及mRNA表达[（0.64±0.03）比（0.52±0.03）]显著减少,P均〈0.05;（3）Wistar心梗组和Wistar心梗＋氯化钴组血糖、Caspase-3光密度值及mRNA表达未见明显差异（P均〉0.05）。结论：慢性氯化钴处理可降低GK糖尿病大鼠血糖,减少Caspase-3凋亡基因在缺血坏死区的表达。

Objective：To explore the influence of chronic cobalt chloride （CoCl2 ） treatment on blood glucose and car-diomyocyte apoptosis in diabetic rats .Methods：In vivo MI model of rat was established using male GK diabetic rats and male Wistar rats （all weighing 200g-250g） .Blood glucose level was measured before model establishment .Af-ter successful operation ,rats were divided into six groups：Wistar sham operation group ,Wistar MI group ,Wistar MI ＋ CoCl2 group ,GK sham operation group ,GK MI group and GK MI ＋ CoCl2 group ,six rats survived in each group at least .After three weeks ,blood glucose level was measured and heart was excised ,expression of Caspase-3 apoptosis gene in ischemic necrosis area was measured .Results：（1） Compared with GK sham operation group and GK MI group on three weeks after operation ,there was significant reduction in blood glucose level [ （27.73 ± 2.58） mmol/L ,（27.87 ± 3.18） mmol/L vs .（16.3 ± 2.15） mmol/L] in GK MI ＋ CoCl2 group ,P〈0.05 both;（2） Compared with GK MI group ,there were significant reductions in optical density [ （0.84 ± 0.03） vs .（0.70 ± 0.03）] and mRNA ex-pression [ （0.64 ± 0.03） vs .（0.52 ± 0.03）] of Caspase-3 in GK MI ＋ CoCl2 group ,P〈0.05 both;（3） There were no significant difference in blood glucose level ,optical density and mRNA expression of Caspase-3 in Wistar MI group and Wistar MI ＋ CoCl2 group , P〉0.05 all .Conclusion：Chronic cobalt chloride treatment can decrease blood glucose level and reduce expression of Caspase-3 apoptosis gene in ischemic necrosis area in diabetic rats .