评估无血清培养子宫内膜间充质干细胞的可行性

Feasibility of serum-free medium in cultivating endometrium-derived mesenchymal stem cell

目的使用无血清培养基体外分离扩增子宫内膜间充质干细胞,研究其生物学特性。方法比较在无血清培养基中和含10%胎牛血清培养基中子宫内膜间充质干细胞细胞形态细胞表型、增殖能力、细胞活率和分化能力等生物学特性。结果无血清培养基和有血清培养基培养的子宫内膜间充质干细胞形态相似,但是前者呈明显的漩涡状生长,更加细长,立体感更强;无血清和有血清培养的子宫内膜间充质干细胞表面标记物均呈阳性;无血清培养的子宫内膜间充质干细胞细胞活率更高、细胞增殖能力更强。结论无血清培养基能够在体外扩增子宫内膜间充质干细胞,并使其生物学特性(细胞增殖,细胞活率)优于胎牛血清培养的子宫内膜间充质干细胞,且分化能力无改变,可以取代胎牛血清用于细胞治疗,避免有血清培养的干细胞治疗引起的人畜共患病及免疫原性反应。

Objective To dissect and amplify the endometrium-derived mesenchymal stem cells （EnMSCs） in serum-free medium （SFM） in vitro and assess their bionomics. Methods Bion0mics of EnMSCs in SFM and serum-containing （fetal bovine serum） medium （SCM）, such as morphology, phenotype, proliferative activity, cell viability and differentiation capability, were compared. Results Similar cell morphology was shown within EnMSCs cultivated both in SFM and SCM, while the EnMSCs cultured in SFM showed obvious swirling growth and stronger three-dimensional effect, which was more slender. Surface markers of EnMSCs cultivated both in SFM and SCM were all positive. Cell viability and cell proliferation were higher in SFM than in SCM. Conclusion According to the results of the present study, we can conclude that EnMSCs can be cultivated in SFM with no changes in their differentiating capacity in vitro, and their bionomics （proliferation and cell viability） are better performed than those cultivated in SCM. Therefore, SFM can be used in cell therapy instead of fetal bovine serum. This finding gives us a hope for future cell therapy studies and trials with little concern about zoonotic infections or immunological reaction.