不同退化湿地中嗜冷甲烷古菌的变化规律研究

Changing Trend of Psychrotrophic Methanogens in Different Degraded Wetlands

该试验通过厌氧分离、滚管纯化技术对同一时期不同退化程度湿地中的嗜冷甲烷古菌进行分离,采用血球计数法对嗜冷甲烷古菌计数,应用荧光显微镜观察其形态特征,并使用气相色谱法对其产甲烷量进行测定分析,依据试验结果分析嗜冷甲烷古菌与高寒湿地退化的联系。结果表明:随着湿地退化程度增加,嗜冷甲烷古菌的种类和数量都有增加,其产甲烷气体量亦有明显增加,对嗜冷甲烷古菌的特性研究可以在一定水平上指示湿地的退化程度。

The experiment is to study psychrophilic methanogens vmiation during the same period of different degraded wetlands in the Tibetan Plateau. Methanogens were separated through anaerobic separation and rolling tube purification technique in the different samples. Blood cell counting method was used to count methanogens, and their morphological characteristics were observed by fluorescence microscopy, the amount of methane was measured by gas chromatography analysis. The relations between alpine wetland degradation and psychrophilic methanogens were analyzed according to the results. Results showed that with the degradation of wetlands increasing, types and quantities of psychrophi^lic methanogens are increasing, and the gas production of psychrophilic vaethanogens also significantly increased. Experiments for psychrotrophic characteristics of methanogens at a level can indicate the extent of degradation of wetlands.