摘要
目的:探讨急性心肌梗死(acute myocardial infarction,AMI)患者血清S100A1表达及临床意义。方法:选择2011年1月至2013年12月收治的AMI患者86例和健康对照30例,采用酶联免疫吸附试验(ELISA)法检测血清S100A1和血浆肌钙蛋白Ⅰ(cardiac troponinⅠ,c TnⅠ)水平;采用荧光免疫方法检测血清高敏C-反应蛋白(high-sensitivity C reactive protein,hs-CRP)和血清肌酸激酶同工酶(creatine kinase-MB,CK-MB)水平。结果:AMI组患者血清S100A1、hs-CRP、c TnⅠ、CK-MB水平明显高于对照组(P〈0.01)。血清S100A1水平与hs-CRP、c TnⅠ、CK-MB呈正相关(P〈0.01或0.05)。AMI患者发病初期血清S100A1水平持续升高,受试者工作特征曲线分析结果显示,曲线下面积为0.855(95%CI:0.814~0.889),以S100A1水平114μg/L为临界值,诊断灵敏度为94.7%,特异度为88.9%。结论:血清S100A1在AMI初期迅速持续的升高,对AMI诊断敏感度性和特异度高,作为新的血清学指标在AMI早期诊断中有重要参考价值。
Objective: To investigate the serum levels of S100A1 in patients with acute myocardial infarction (AMI) and its clinical significance. Methods: The level of serum S100A1 and plasma cardiac troponin I (cTn I ) level in 86 patients with AMI and 30 control volunteers were detected by ELISA, and serum high-sensitivity C reactive protein (hs-CRP) and creatine kinase-MB (CK-MB) were detected by fluorescence immunoassay. Results: The levels of S100A1, hs-CRP, cTn I and CK-MB in patients with AMI group were significantly higher than those in the control group (P〈0. 01 ). A significant positive correlation was found be- tween the serum level of S100Alwith hs-CRP, cTn I , CK-MB (P〈0. 01 or 0.05). Serum S100A1 level continued to rise in the ear- ly onset of AMI. ROC analysis showed that the area under the curve (AUC) was 0. 855 (95%C1: 0. 814-0. 889) , the best S IOOA1 critical level was 114 μg/L , the diagnostic sensitivity was 94. 7% and the specificity was 88.9%. Conclusions: Serum S100A1 rap- id sustained elevates in the early of AMI. It is high sensitivity and specificity for diagnosis of AMI, and it can be used as a new sero- logical indexes in the diagnosis of early AMI, and it has an important reference value.
出处
《沈阳医学院学报》
2015年第2期94-96,共3页
Journal of Shenyang Medical College