心绞痛贴膏穴位贴敷预处理对急性心肌梗死大鼠血清心肌酶及TNF-α、IL-6的影响

Experimental Study on the Influence of Angina Pectoris Patch Acupoint Application Pretreatment on Acute Myocardial Infarction in Rats in Serum Myocardial Enzyme,TNF-α,and IL-6

目的观察＂心绞痛贴膏＂穴位贴敷预处理对急性心梗大鼠的干预效应及可能的作用机制。方法健康Wistar大鼠55只随机分为正常组、模型组、对照组和治疗组,正常组10只,模型组、对照组和治疗组每组15只。在清洁级实验室以普通饲料饲养。对照组于胸前区贴硝酸甘油贴膜0.3 mg,治疗组予心绞痛贴膏穴位贴敷于心俞、膻中、神阙、后三里穴（每穴0.1 g）,每日1次,每穴1贴,维持4~6 h,疗程10 d。模型组、对照组、治疗组均每日2 mg/kg多点腹腔注射异丙肾上腺素（ISO）10 d。第10日于实验结束时,查各组大鼠心电图,以ST段明显上抬或下移为造模成功的初步判断标准,取腹主动脉光镜下观察心肌病理学形态,检测大鼠肌酸激酶（CK）、肌酸激酶同工酶（CK-MB）、乳酸脱氢酶（LDH）、肿瘤坏死因子-α（TNF-α）、白介素-6（IL-6）的含量。结果病理结果显示,对照组和治疗组心肌细胞损伤坏死较模型组轻,但也可见少量断裂、缺损及空白区着色不均匀出现。模型组心肌细胞坏死严重。治疗组和对照组的心肌酶学水平、TNF-α、IL-6表达显著低于模型组（P〈0.01）。结论心绞痛贴膏穴位贴敷预处理能够减轻AMI大鼠心肌损伤坏死程度,并可能通过抑制大鼠TNF-α、IL-6的表达减少炎症细胞浸润,从而起到改善心肌缺血的作用。

Objectives： To discuss the effect of angina pectoris patch sticking preconditioning on acute myocar- dial infarction in rats and its possible mechanism. Methods： 55 healthy Wistar rats were randomly divided into the normal group,model group,control group and treatment group,10 rats in the normal group,15 rats in the model group,control group and treatment group,fed with normal diet in a clean laboratory. The control group patched nitroglycerin 0.3 mg on the chest area,while the treatment group were treated with angina pectoris plaster acupoint application in Xinshu, Tanzhong, Shenque, after three points （each point 0.1 g）, once a day, every point 1 sticker,maintaining 6 h. The treatment lasted for10 days. The model group,the control group,the treatment group received daily multipoint intraperitoneal injection of isoproterenal （ISO） 2 mg/kg for 10 days. On the tenth day at the end of experiment,the ECG of the rat was examined,and the success of modeling was preliminary judged by ST segment elevation or depression, and the myocardial pathological morphology of abdominal aorta was observed under light microscope to observe, detecting the content of rat CK, CK-MB,LDH,TNF- alpha,lL-6. Results： The pathological results showed that myocardial cell damage and necrosis of the treatment group and the control group was lighter than that of the model group,but a small amount of fault was 0bvious,and defect and blank re- gion coloring appeared. Myocardial cell necrosis in the Model group was serious. The myocardial enzyme levels of TNF-alpha, IL-6 of the treatment group and control group were significantly lower than those of the model group （P〈0.01）. Conclusion： Angina piaster acupoint application of pretreatment can reduce the degree of necrosis of myocardial injury in AMI rats,and may inhibit the TNF-alpha,the expression of IL-6 of the rat,reducing the in- filtration of inflammatory cells, so as to improve myocardial ischemia.