血清乙型肝炎病毒外膜大蛋白188例检测结果分析

Analysis of 188 cases of serum hepatitis B virus large surface protein

目的分析188例血清乙型肝炎病毒外膜大蛋白(LHBs)检测结果,探讨LHBs的临床应用价值及其与乙型肝炎病毒DNA(HBV-DNA)、乙型肝炎病毒e抗原(HBe Ag)之间的相关性。方法用酶联免疫吸附试验(ELISA)法定量检测乙型肝炎病毒标志物、LHBs,采用实时荧光聚合酶链反应(PCR)定量检测HBV-DNA。结果在相同的乙型肝炎病毒标志物模式中,HBV-DNA与LHBs检测结果的阳性率相关(r=0.891,P=0.001)。HBe Ag阳性患者血清的检测结果显示,LHBs测定值与HBe Ag测定值相关(r=0.267,P=0.019);乙型肝炎病毒标志物阴性及其余乙型肝炎病毒标志物模式LHBs测定值与HBe Ag、HBV-DNA测定值均不相关(P>0.05)。结论 LHBs与HBV-DNA阳性率具有一致性,能够反映乙型肝炎患者体内病毒复制情况,可以视作HBe Ag阴性患者中病毒复制的一个新的血清学指标,临床应用中应将LHBs与HBs Ag及HBV-DNA联合检测。

Objective To explore the value of clinical application of （hepatitis B virus large surface protein, LHBs） and the correlation between LHBs and HBV-DNA, or LHBs and hepatitis B virus e antigen（HBeAg） by analyzing 188 cases of serum LHBs. Methods HBV markers and LHBs were quantified by using enzyme linked immune-sorbent assay （ELISA） method, HBV-DNA were quantified by the real-time fluorescence polymerase chain reaction （PCR）. Results In the same hepatitis B virus markers pattern, the positive rates of HBV-DNA and LHBs were correlated （r=0.891） and the difference was statistically significant （P=0.001）. LHBs was only correlated to HBeAg in patients with HBeAg positive （r=0.267,P=0.019）. No significant correlation were found between LHBs and HBeAg or HBV-DNA in patients with all HBV markers negative pattern （P〉0.05）. Conclusion The positive rate of LHBs was consistent with HBV-DNA, which may reflect the virus replication in patients with HBV infection. It can be regarded as a new serologic indicator of virus replication in patients with HBeAg negative and should be detected combined with HBsAg and HBV-DNA.