摘要
采用复合磷脂酶在水相体系中酶解大豆卵磷脂制备甘油磷脂酰胆碱(GPC),磷脂酶A1和磷脂酶A2复合酶解作用,不仅有效地缩短了酶解时间,更能提高GPC的得率。通过响应面优化试验确定最佳酶解条件为:底物质量浓度61 g/L,酶解温度45.2℃,磷脂酶A1添加量16.86 U/m L,磷脂酶A2添加量21.98 U/m L,Ca Cl2添加量5.18 g/L。在最佳酶解条件下酶解2 h,GPC得率平均值为96.25%。
Glycerylphosphorylcholine (GPC) was prepared by the enzymatic hydrolysis of soybean lecithinin aqueous media with complex phospholipase as catalyst, and the complex enzymatic hydrolysis by phos-pholipase A1 and phospholipase A2 could not only effectively shorten the enzymolysis time, but also im-prove the yield of GPC. The optimal enzymatic hydrolysis conditions were determined by response surfacemethodology as follows : mass concentration of substrate 61 g/L, enzymolysis temperature 45.2 ℃ , dosageof phospholipase A 16.86 U/mL, dosage of phospholipase A2 21.98 U/mL, dosage of CaC12 5.18 g/L.Under the optimal conditions, the average yield of GPC was 96.25 % after enzymatic hydrolysis for 2 h.
出处
《中国油脂》
CAS
CSCD
北大核心
2015年第6期57-61,共5页
China Oils and Fats
关键词
复合磷脂酶
水相体系
甘油磷脂酰胆碱
酶法制备
complex phospholipase
aqueous media
glycerylphosphorylcholine
enzymatic preparation