罗哌卡因联合苏芬太尼对神经病理性疼痛大鼠镇痛作用的实验研究

Experimental study of the effect of ropivacaine combined with sufentanil analgesic on neuropathic pain in rats

目的：探讨罗哌卡因和苏芬太尼联合用药对神经病理性疼痛大鼠的镇痛效果。方法采用慢性坐骨神经缩窄性损伤方法造大鼠神经病理性疼痛模型，按照上述造模方法一共制造40只神经病理性疼痛大鼠，随机分成 A 组（罗哌卡因和苏芬太尼联合给药，n ＝10）、B 组（罗哌卡因给药，n ＝10）、C 组（苏芬太尼给药，n ＝10）和 D 组（不给药，n ＝10）。纳入10只健康的大鼠不进行任何处理，正常喂养，命名为 E 组。在手术后1、3、7、11、14、21 d 用 von-Frey filaments 测定机械性痛觉过敏（MWT），按 Hargreaves 法测定热痛觉过敏（TWL）。采用 ELISA 试剂盒（IL-6和 TNF-α）检测手术前和术后1、7、14、21 d 时血清中 IL-6和 TNF-α浓度。结果手术后1 d 的 MWT 和 TWL 与手术前差异均无统计学意义（均 P ＞0．05）。A 组、B 组和 C 组术后3、7、11、14和21 d MWT 和 TWL 均显著低于手术前和手术后1 d 时的值（均 P ＜0．05），并且 A 组术后3、7、11、14和21 d MWT 和 TWL 均显著低于 B 组和 C 组术后3、7、11、14和21 d 时的值（均 P ＜0．05）。A 组术后7、14和21 d 血清 IL-6浓度和 TNF-α浓度均显著低于 B 组和 C 组术后7、14和21 d 血清 IL-6浓度（t ＝7．10、5．84、9．11，均 P ＜0．05）。结论罗哌卡因联合苏芬太尼对神经病理性疼痛大鼠镇痛作用显著高于罗哌卡因或苏芬太尼单独治疗。

Objective To explore the effect of the ropivacaine combined with sufentanil on neuropathic pain in rats.Methods The constriction injury method was performed to establish rat neuropathic pain model and 40 neu-ropathic rats were produced in accordance with the above modeling methods,which were divided into group A（ropiva-caine and sufentanil combined administration,n =10）,group B（ropivacaine administration,n =10）,group C（sufen-tanil administration,n =10）and group D（no administration,n =10）.A total of 10 healthy rats without any treatment, the normal feeding,named as group E.At 1,3,7,11,14,21d after operation von -Frey filaments determination of mechanical hyperalgesia（MWT）,and the Hargreaves method for the determination of thermal hyperalgesia（TWL） were measured and the ELISA Kit （IL -6 and TNF -α） were used to detect serum IL -6 and TNF -αconcentrations.Results Before and at 1 day after the operation mechanical allodynia and thermal hyperalgesia and allergy treatment had no significant difference（all P 〉0.05）.Mechanical allodynia and thermal hyperalgesia of group A,B and C were significantly lower than those before the treatment and at 1 day after operation（all P 〈0.05）,and mechanical hyperalgesia and thermal hyperalgesia of group A was significantly lower than those of group B and C （all P 〈0.05）.The serum IL -6 and TNF -αconcentrations of 7,14 and 21 days in group A were significantly lower than those of group B and C（t =7.10,5.84,9.11,all P 〈0.05）.Conclusion The effects of ropivacaine combined with sufentanil analgesic on neuropathic pain of rats was significantly higher than that of ropivacaine or sufentanil alone in the treatment.