黑米种皮色素的遗传分析和分子标记

Genetics Regularity and Molecular Marker of Pericarp Pigment in Black Rice

为明确黑米种皮色素的遗传基础,利用籼稻黑米黑B和无色东北粳稻杂交,获得F1和F2分离群体。F1种皮表现黑色,说明种皮中的黑色性状受显性基因控制。同时,其F2代后代表现3:1的孟德尔分离比,表明黑色性状基因是受一对显性基因控制,暂定名为BP(black pericarp)。利用F2分离群体作为定位群体,用均匀分布在水稻12条染色体上的128对SSR引物和45对indel引物进行BSA和分子标记分析,利用MAPMAKER3.0软件进行连锁分析,将控制黑色性状基因定位在第4染色体上。目的基因BP位于引物R4M23和R4M43之间,遗传距离分别为10.2c M和14.6c M。

The genetic regularity and molecular marker of pericarp pigment in black rice were investigated by analyzing the F1 and F2 populations from the crosses of black periearp line （Hei B）xwhite pericarp line originated from northeast of China. The results showed that plants were all black pericarp in the F1 population. While in the F2 populations, the segregation ratio of black periearp plants and white pericarp plants was 3 ： 1. This suggests that the trait of black pericarp was completely controlled by one dominant gene. The gene controlling black pericarp trait, designated BP, was mapped in chromosome 4 based on BSA analysis and linkage mapping using 128 SSRs and 45 INDELs scattered on whole chromosomes. The BP was located between R4M23and R4M43, with a distance of 10.2cM and 14.6cM, respectively.