一种高纯度提取草地土壤微生物DNA方法的建立

A High Purity Method for DNA Extraction from Soil Microorganisms of Grassland

本文研究建立一套高效、简便的草地土壤微生物DNA的提取和纯化方法。提取的微生物总DNA片段大小在23 kb左右,纯化后的DNA产量为(7.76±1.53)μg·g-1,OD260/OD280为1.78±0.04,OD260/OD230为1.76±0.02。

The aim of the study was to establish a highly efficient, simple method to extract and purify soil mi- crobial DNA. The extraction of microbial DNA fragment was about 23 kb in size, the yield of DNA solution after pu- rification was（7.76±1.53）μg·g^-1,OD260/OD280 was1.78±0.04,OD260/OD230 was1.76±0.02.