结肠癌患者血清肿瘤标志物Imp1、p62、Koc、PTN、CCSA-2检测及临床价值

Serum tumor markers Imp1,p62,Koc,PTN,CCSA-2levels of colon cancer patients

目的：研究结肠癌患者血清中肿瘤标志物Imp1、p62、Koc、PTN、结肠癌特异性抗原-2（CCSA-2）含量及临床价值。方法：将2013年5月~2014年6月期间在我院接受手术切除治疗的80例结肠癌患者纳入研究的观察组,同期在我院接受手术切除治疗的40例结肠腺瘤患者纳入研究的结肠腺瘤组,在我院体检的健康者40例纳入研究的健康对照组,检测血清中Imp1、p62、Koc、PTN、CCSA-2含量以及肿瘤组织中促凋亡基因、促增殖基因的mRNA含量。结果：（1）观察组患者血清中Imp1、p62、Koc、PTN、CCSA-2的含量高于结肠腺瘤组和健康对照组（P〈0.05）;（2）不同TNM分期患者的血清肿瘤标志物含量有差异,且TNM分期越高,血清中Imp1、p62、Koc、PTN、CCSA-2的含量越高（P〈0.05）;（3）结肠癌组织中MTS1、Caspase-3、Bax含量低于结肠腺瘤组织,且与血清肿瘤标志物呈负相关;Bcl-2、Survivin、Livin含量高于结肠腺瘤组织,且与血清肿瘤标志物呈正相关。结论：结肠癌患者血清中肿瘤标志物Imp1、p62、Koc、PTN、CCSA-2含量异常升高,且与肿瘤组织中促凋亡基因、促增殖基因的表达具有相关性。

Objective： To study the contents of serum tumor markers Impl, p62, Koc, PTN, CCSA-2 of colon cancer patients. Methods： A total of 80 cases of colon cancer received surgery recection treatment in our hospital from 2013 May to 2014 June were enrolled in observation group; 40 cases of colon adenoma patients received surgical excision in our hospital were enrolled in colon adenomagroup; 40 cases of healthy people received health examination in our hospital were enrolled in healthy control group. Then serum tumor markers Impl, p62, Koc, PTN, CCSA-2 contents and mRNA contents of apoptosis gene, proliferation gene were assayed. Results.. Serum tumor markers Imp1, p62, Koc, PTN, CCSA-2 contents of observation group were significantly higher than those of colon adenoma group and healthy control group （P 〈 0. 05）. There were significant differences in serum tumor markers contents among patients at different TNM stages（P〈0.05）. The higher TNM stage was, the higher serum Impl, p62, Koc, PTN, CCSA-2 contents were. MTS1 ,Caspase-3, Bax contents in colon cancer tissue were significantly lower than those of colon adenoma and they were negatively correlated with serum tumor marker. Bcl-2, Survivin, Livin contents were higher than those of colon adenoma and were positively correlated with serum tumor marker. Conclusion： Serum tumor markers Impl, p62, Koc, PTN, CCSA-2 contents of colon cancer patients show abnormally increase; and it is correlated with apoptosis and proliferation of gene.