三参汤对早期糖尿病肾病大鼠血清血管紧张素Ⅱ、VEGF和尿ET-1含量的影响

Influence of Sanshen Decoction on the levels of angio tensin Ⅱ,Endothelin-1,Vascular endothelial growth factor in rats with early diabetic nephropathy

目的观察三参汤对早期糖尿病肾病大鼠血清血管紧张素Ⅱ(ATⅡ)、血管内皮生长因子(VEGF)、血浆和尿内皮素-1(ET-1)的影响,探讨三参汤治疗早期糖尿病肾病的作用机制。方法采用高脂饲养+链脲佐菌素注射方法复制2型糖尿病肾病大鼠模型,造模成功大鼠随机分为模型组、对照组和治疗组各10只,另选10只尾静脉注射等量生理盐水大鼠作为空白组。模型组和空白组灌胃等体积生理盐水,对照组灌胃厄贝沙坦,治疗组灌胃三参汤,灌胃3周。治疗前后酶免法检测4组大鼠血清ATⅡ、VEGF、血浆和尿ET-1浓度,化学法检测尿微量白蛋白、血糖浓度。结果治疗后治疗组大鼠血清ATⅡ、VEGF和尿中ET-1浓度及血糖、尿微量白蛋白水平均明显低于模型组(P均<0.05)。结论三参汤可能通过调节肾素-血管紧张素系统而发挥治疗糖尿病肾病作用。

Objective It is to observe the influence of Sanshen Decoction on angio tensin Ⅱ（ ATⅡ）,Endothelin-1（ ET-1）,Vascular endothelial growth factor（VEGF）levels of rats with early diabetic nephropathy,and investigate the mecha-nism in treatment of early diabetic nephropathy. Methods The rat models of type 2 diabetic nephropathy were duplicated by u-sing high-fat diet and streptozotocin and divided into model group,control group and treatment group with 10 cases in each. Other 10 rats by tail vein injected with saline were selected as the blank group. The animals were treated by intragastric admin-istration for equal volume of saline in model group and blank group,irbesartan in control group and Sanshen Decoction in treat-ment group,the treatment kept for 3 weeks. Before and after treatment,the levels of ATⅡ,ET-1,and VEGF were detected by ELISA method,and the levels of urine micro albumin and blood sugar were detected by chemical turbidimetry. Results Af-ter the treatment of Sanshen Decoction,the levels of ATⅡ,ET-1 and VEGF were decreased,and the level of blood sugar and urine micro albumin were reduced,there were significant differences between treatment group and model group（ all P ﹤0. 05）. Conclusion Sanshen Decoction might play the therapeutic role on diabetic nephropathy through regulating the renin-angiotensin system.