几种可利用多氯联苯和多溴联苯醚植物内生菌的分离及初步鉴定

Isolation and identification of plant endophytic bacteria survived in polychlorinated biphenyls and polybrominated diphenyl ethers

采用PDA、NA以及高氏一号培养基,分别从毛白杨、绦柳、荻以及二穗短柄草植株内分离、纯化出37株内生菌,其中34株为细菌,3株为放线菌.以分离纯化得到菌株各自对应的营养培养基并添加不同浓度的污染物对获得的37株内生菌进行污染物的耐受筛选,发现24株分别对20 mg·L-1的aroclor1254和BDE209具有一定的耐受性.利用无机盐培养基并添加污染物作为微生物唯一碳源筛选,发现菌株CPY-4和菌株SGL-1可以在只提供4-BDE的无机盐培养基中存活,菌株CPY-4,SGL-1、菌株4、菌株13可以在提供BDE209和aroclor1254的无机盐培养基中存活.形态学观察和16Sr DNA序列同源性分析,初步确定菌株SGL-1为克雷伯氏菌属,菌株CPY-4为地衣芽胞杆菌,菌株4和菌株13为肠杆菌属.

Thirty seven endophytic bacteria were isolated from Populus tomentosa,Salix matsudana f. pendula,Triarrherca sacchariflora and Brachypodium distachyon by PDA,NA and Trypticase Soy Broth media. Three strains were actinomycetes and the other 34 ones belonged to bacteria. Twenty four endophytic bacteria were founded to tolerate 20 mg·L- 1BDE209 or aroclor1254 in each corresponding culture media including PDA,NA and Trypticase Soy Broth meida. The strain CPY-4and the strain SGL-1 could use 4-BDE as their carbon source in each inorganic salt culture media respectively. Four strains,CPY-4,SGL-1,4 and 13 could utilize the BDE209 and aroclor1254 jointly supplied in each inorganic salt culture media. Morphological observation and the 16 Sr DNA sequence analysis showed the strain 4 and the strain 13 were Enterobacter sp.,the strain SGL-1 was Klebsiella sp. and the strain CPY-4 was Bacillus licheniformis.