银芷肛肠熏洗剂对痔组织细胞因子VEGF、CD68及MMP9的影响

The Effect of Yin Zhi Anorectal Smoked Lotion Acting on the Hemorrhoid Tissue Cytokines Factor VEGF、CD68 and MMP9

目的观察银芷肛肠熏洗剂对痔组织VEGF、CD68、MMP9的影响。方法选取痔急性发作期患者40例,随机分为治疗组和对照组各20例。治疗组予银芷肛肠熏洗剂,对照组予金玄痔科熏洗散,肛门局部熏洗连续治疗后7d,取痔组织进行免疫组化染色检查,并与未用药直接手术痔患者(空白组)的组织进行对比,观察药物对痔组织VEGF、CD68、MMP9的影响。结果给药后银芷肛肠熏洗剂治疗组及金玄痔科熏洗散对照组抑制VEGF、CD68、MMP9表达的作用均优于空白组(P<0.01);银芷肛肠熏洗剂抑制VEGF、CD68、MMP9的作用优于金玄痔科熏洗散对照组(P<0.01/0.05)。结论银芷肛肠熏洗剂能抑制痔组织VEGF、CD68、MMP9的表达,其对痔的治疗作用可能与抑制VEGF、CD68、MMP9表达有关。

Objective To observe effect of YinZhi anorectal smoked lotion acting on the hemorrhoid tissue cytokines factor VEGF、CD68 and MMP9. Methods Selected 40 cases of acute episode of hemorrhoid patients were randomly divided into 20 patients in each treatment group and the control group. The treatment group were cured with YinZhi anorectal smoked lotion and the control group were cured with Ma Ying-Long Jin Xuan hemorrhoid washing powder,All two group were fumigated partialy on anal after 7 consecutive days. The hemorrhoid tissue gotten from patients compare with that were cured through surgery directly without medication by immunohistochemical staining examination. Observe the effect of medication on hemorrhoid tissue cytokines factor VEGF、CD68 and MMP9. Results After medical treatment,YinZhi anorectal smoked lotion treatment group and Ma Ying-Long Jin Xuan hemorrhoid washing powder control group is better than blank group about inhibiting VEGF,CD68,MMP9 expression（P〈0.01）;YinZhi anorectal smoked lotion treatment group is better than Ma Ying-Long Jin Xuan hemorrhoid washing powder control group（P〈0.01/0.05）. Conclusion YinZhi anorectal smoked lotion inhibits expression of hemorrhoidal tissue cytokines factor VEGF、CD68 and MMP9. The therapeutical effect of YinZhi anorectal smoked lotion acting on hemorrhoidal tissue is possibly related with expression of inhibiting VEGF,CD68,MMP9.