低氧下大鼠AQP5基因差异性表达

HYPOXIA EXPOSURE CAUSED DIFFERENT AQP5 GENE EXPRESSIONS IN RATS

目的检测低氧下大鼠肺组织AQP5表达水平的改变,探讨AQP5表达量变化与低氧下肺水肿发病的分子机制。方法构建低氧大鼠模型;采用荧光定量PCR技术检测低氧下大鼠肺组织中AQP5 mRNA表达水平;采用Western-Blot技术检测低氧下大鼠肺组织中AQP5蛋白表达水平;并与平原对照组进行表达差异性比较。结果低氧组大鼠肺组织中AQP5 mRNA表达水平随着时间的延长升高,其中72小时组AQP5 mRNA表达水平达到最高,且差异极为显著(P<0.01);并且低氧组大鼠肺组织中AQP5蛋白表达水平变化与其mRNA表达水平变化基本一致。结论 AQP5在机体高原习服过程中起到了重要的调节过程;AQP5对肺泡性肺水肿发生起代偿作用;机体进入高原环境,肺组织中AQP5表达量最高值可作为机体发生高原肺水肿的诊断标准。

Objective To study the mRNA expression levels of AQP5 in lung tissue of rats with acute,chronic exposed hypoxia in order to clarify the relationship between the expression changes of AQP5 and pulmonary edema, to provide the theoretical basis for the study of high altitude adaptation mechanisms. Methods The acute, chronic exposed hypoxic rat model were set up, at the same time, we observed the change of the expression level of AQP5 mRNA in lung tissue by quantitative PCR in the acute and chronic hypoxia;then the AQP5 protein expression levels were detected by Western - Blot. Results the mRNA expression levels of AQP5 genes were increased significantly both in the acute and chronic hypoxia group（ P 〈 0.01 ）. In addition, after 72 hour AQP5 mRNA expression level has reached the significant highest levels（ P 〈 0. 001 ） ;AQP5 protein expression level was consistent with the mRNA levels. Conclusion AQP5 plays important role in adaptation mechanisms in high altitude;AQP5 also play a compen- satory role in the pathogenesis of the pulmonary edema when exposed to acute and chronic hypoxia,it might also be a potential diagnosis values for the severity of the high altitude pulmonary edema.