HPLC-DAD波长切换法同时测定青钱柳提取物中5种有效成分

Simultaneous Determination of Five Active Components in Cyclocarya Paliurus( Batal. )Iljinshaja Extractive by HPLC- DAD Wavelength Switching

目的：建立HPLC—DAD波长切换技术同时测定青钱柳提取物中异槲皮苷、槲皮素、山奈酚、山奈素、熊果酸5种有效成分的含量测定方法。方法：采用ThermoSyncronisC，8色谱柱（250mm×4．6mm，5μm）；流动相为乙腈-0．2％磷酸水溶液，流速1．0mL·min^-1柱温35℃，进样量20仙L，异槲皮苷、槲皮素、山奈酚、山奈素检测波长为360Irlm，熊果酸检测波长为210nm。结果：醇提物中各成分含量为0．6485％、0．0467％、0．0148％、0．0017％、0．0359％，水提物中异槲皮苷含量为0．0952％。异槲皮苷、槲皮素、山奈酚、山奈素、熊果酸的质量浓度分别在15．625—625μg·mL^-1（r=0．9996）、4．05～162μg·mL-1（r=0．9998）、0．66～66μg·mL^-1（r=0．9999）、0．09～28．8μg·mL^-4（r：0．9998）、2．87-114．8μ·mL^-1（r=0．9998）内与色谱峰峰面积．呈良好的线性关系，平均回收率（n=6）均在100．24％、99．07％、101．52％、102．11％、97．24％（n=6）。结论：75％乙醇提取青钱柳中成分，基本可提取完全，残渣再用水提后只有微量的异槲皮苷；文章所建立的同时测定青钱柳中5种成分的含量，该方法简便、准确，具有较好的重复性和稳定性，为青钱柳盾量的全面评价提供了科举依据．

Objective：To establish a method for the simultaneous determination of five active components of isoquercitrin,quer- cetin, kaempferol and kaempferidel, ursolic acid in Cydocarya paliurus （Batal.） Iljinshaja extractive. Methods：The Thermo Syn- cronis C ls （250 mm × 4.6mm,5 μm）was adopted with the mobile phase of 0. 2% phosphoric acid solution- acetonitrile. The flow rate was 1.0mL ~ min-1 ,the column temperature was 35 ~C and the injection volume was 20 μL. The detecting wavelength of iso- quercitrin, quercetin, kaempferol, kaempferidel was 360 nm ; the detecting wavelength of ursolic acid was 210 nm. Results ： Each component content in alcohol extract was 0.6485% ,0. 0467% ,0. 0148% ,0. 0017% and 0. 0359%. The content of isoquercitrin in aqueous extract was 0. 0952%. The linear ranges for isoquercitrin, quercetin, kaempferol, kaempferidel and ursolic acid were 15. 625 - 625 μ · mL -1 （ r = O. 9996） ,4.05 - 162 μg · mL -1 （ r = 0. 9998 ） ,0.66 -- 66 μg · mL - 1 （ r = O. 9999 ） ,0.09 - 28.8 μg · mL-1 （ r = 0. 9998 ） and 2. 87 - 114. 8μg · mL-1 （ r = 0. 9998 ）, respectively; and the average recoveries （ n = 6 ） were 100. 24% ,99.07 %, 101.52% , 102. 11% and 97.24% , respectively （n = 6）. Conclusion：The composition of CycIocarya paliurus （Batal） Iljinsk with 75% alcohol extract basic can be extracted completely;residue with water extraction only a trace of isoquer- eitrin. The method is highly simple, accurate with good repeatability and stability, which can provide the basis for evaluating the quality of Cyclocarya paliurus（Batal） Iljinsk.