酶法制备稻壳木聚糖的研究

Enzymatic Production of Xylan from Rice Hull

采用α-淀粉酶和碱性蛋白酶分别降解稻壳中的淀粉和蛋白质,来制备稻壳木聚糖。在淀粉的酶解中,通过实验分析得出最佳酶解条件为:料液比1∶12 g/m L,水解温度100℃,加酶量1.5%稻壳淀粉,反应时间20 min。稻壳中淀粉的含量从21.52%降至0.72%。在蛋白质的酶解中,通过实验分析得出最佳酶解条件为:料液比1∶12 g/m L,反应温度50℃,加酶量3%稻壳蛋白,酶解时间120 min。酶解后稻壳中蛋白质残留量为2.97%。经过去淀粉和蛋白质的稻壳中木聚糖含量为46.68%。

Starch and protein in rice hull were degraded by α-amylase and alkaline protease respectively, then xylan were produced from rice hull. The optimal experimental conditions for hydrolyzing starch are： the temperature was 100℃ ,the ratio of stuff and liquid was 1 ： 12 g ： mL, digestion time was 20 min,and enzyme concentration was 1.5 % wheat bran starch. The remained content of starch is reduced to 0.72 % from original 21.52 %. The optimal experimental conditions for hydrolyzing protein are ： the temperature was 50℃, the ratio of stuff and liquid was 1 ： 12 g ： mL, digest time was 120 min, and enzyme concentration was 3 % wheat bran protein.The remained content of protein in destarched rice hull is 2.97 %. The destarched and deproteined rice hull contents 46.68 % xylan.