超声诱导提取大豆中脂肪氧合酶的工艺研究

Extraction of Lipoxygenase from Soybean by Ultrasonic Treatment

应用超声诱导法对大豆中脂肪氧合酶进行了粗提,并考察了超声温度、时间和固液比对提取大豆中脂肪氧合酶的影响,并通过正交试验进一步优化实验条件;结果表明,大豆中脂肪氧合酶提取单因素影响最佳工艺条件为超声温度35℃、时间25 min、固液比1∶25 g∶m L;正交实验最佳工艺条件为超声温度30℃,时间30 min,固液比1∶30 g∶m L,此条件下所得酶活为4 033 U/m L,因此这种方法更简单迅速,提取率也更高。

The influence factors including extraction temperature, time and sohd-to-liquid ratio were studied by single factor test and orthogonal test to obtain the optimum conditions of extracting lipoxygenase in soybean by ultrasonic Treatment. The optimum conditions of extracting lipoxygenase in soybean, determined by single factor test, were as follows ： extraction temperature 35℃, time 25 min and solid-to-liquid ratio 1 ： 25 g ： mL. On the basis of the single factor test, orthogonal test was carried out and the results showed that the optimum conditions were extraction temperature 30℃, time enzyme activity was 4 033 U/mL. It was simple treatment, and the exaction rate was also higher. 30 min and solid-to-liquid ratio 1 ： 30 g ： mL, on which the and fast to extract lipoxygenase in soybean by ultrasonic