水溶性丁二酸酐酰化壳聚糖纳米凝胶的制备及其pH响应性研究

Preparation of Water-Soluble Succinyl Chitosan Nanogel and study on Its pH-Responsive Property

以水为介质、碳酸钠为催化剂,室温下制备了水溶性丁二酸酐酰化壳聚糖,研究了反应物料比对产物酰化取代度及水溶性的影响。红外光谱分析表明丁二酸酐成功接枝到了壳聚糖分子链上;XRD结果表明酰化反应改变了壳聚糖的结晶结构;Zeta电位分析发现存在1个等电点：pI=3.54。将丁二酸酐酰化壳聚糖加入到甲酸溶液中,通过自组装法制备纳米凝胶粒子。SEM照片显示该纳米凝胶粒子呈球形,粒径为20～30nm;DLS测试表明,随溶液pH值的增大,粒径从70～80nm增大到350～700nm,表明丁二酸酐酰化壳聚糖纳米凝胶粒子具有一定的pH响应性。

The water-soluble succinyl chitosan was prepared at room temperature using water as medium and sodium carbonate as catalyst.The effects of material ratio on acylated substitution degree and water-solubility were studied.FTIR Results showed that succinic anhydride was successfully grafted onto chitosan.XRD Results indicated that the crystal structure of chitosan was changed after succinic anhydride acylating.Zeta Potential analysis found an isoelectric point：pI=3.54.Nanogel particles were prepared through adding succinyl chitosan into formic acid solution by self-assembly method.SEM Images showed nanogel particles were spherical with particle size of 20～30nm.DLS Tests showed that,particle size increased from 70～80nm to 350～700nm with the increase of pH value,indicated succinyl chitosan nanogel particles had pH-responsive property.