摘要
目的:探讨Hedgehog信号通路三个关键蛋白Patched-1、Gli-1以及Smo在尖锐湿疣患者发病中的作用。方法:本组共收集56例尖锐湿疣患者、40例尖锐湿疣治疗患者以及20例正常人,应用ELISA、Western以及RT-PCR法检测尖锐湿疣皮损患者、咪喹莫特治疗一个疗程后观测患者及正常人皮肤中Patched-1、Gli-1以及Smo的蛋白及mRNA的表达特点。结果:三组Patched-1、Gli-1以及Smo三个指标ELISA以及Western蛋白、RT-PCR mRNA表达,患者组均明显高于对照组(P<0.05),同时治疗组三个蛋白和mRNA表达略高于正常组。结论:尖锐湿疣皮损表皮中Patched-l、Gli-1以及Smo均处于高表达状态,今后或许可用为检测药物治疗后评价指标。
Objectives: To investigate the influence of three proteins, Patched - 1, Gli - 1 and Smo, in the Hedgehog signaling pathway on genital warts patients with lesions. Methods : ELISA, Western and RT - PCR were used to detect Patched - 1, Gli - 1 and Smo proteins and the expression of mRNA of genital warts patients with the lesions treated with imiquimod. The result was compared with that of normal people. Results: There were 56 cases of genital warts untreated patients, 40 cases of genital warts in treatment group and 20 cases in the control group.The level of three key proteins, Patched - 1, Gli - 1 and Smo of the patient group were significantly higher than these of the normal group as well as the expression of mRNA ( P 〈 0. 05). Conchmion: Patched - 1, Gli - 1 and Smo of genital warts patients with lesions are in high expression status and perhaps can be applied on drug treatment evaluation in the future.
出处
《中国性科学》
2015年第6期72-75,共4页
Chinese Journal of Human Sexuality