响应面法优化高压均质提取椪柑渣中可溶性膳食纤维及抗氧化活性研究

Optimization of High-Pressure Homogenization Extraction of Soluble Dietary Fiber from Ponkan Residue Using Response Surface Methodology and Its Antioxidant Activities

以椪柑渣为试验原料,采用响应面分析法(RSM)建立了高压均质(HPH)提取椪柑渣中可溶性膳食纤维(SDF)得率的二次多项数学模型,验证了数学模型的有效性,探讨了均质压力、处理次数和物料温度对SDF得率的作用规律,优化提取工艺参数。结果表明:在压力37 MPa,处理8次,物料温度41℃条件下,SDF提取率高达43.86%。在最佳高压均质工艺条件下,经均质改性的可溶性膳食纤维(H-SDF)具有一定的还原能力,对·OH、O2-·和DPPH均表现出较强的清除能力,其IC50分别为7.89,6.87,6.17 mg/m L。采用高压均质提取椪柑渣中的SDF切实可行。

Response surface methodology （RSM） was employed to optimize the process parameters of high-pressurehomogenization （HPH） extraction of soluble dietary fiber （SDF） from ponkan residue. The adequacy of the model equa- tion for predicting the optimum response values was verified effectively by the validation. Effects of pressure, treatment time and material temperature on yield of SDF were investigated. Maximum yield of SDF from the mathematical model was predicted to be 43.86% dry basis under the condition of pressure 37 MPa, 8 times and material temperature 41 ℃. Under optimal conditions, H-SDF exhibited reducing power, and revealed strong scavenging activity against hydroxyl and superoxide anion radicals and DPPH radicals with an ICs0 of 7.89 mg/mL, 6.87 mg/mL and 6.17 mg/mL, respectively. Therefore, it was feasible to use HPH method to achieve the extraction of the SDF from ponkan pomace.