葡萄酒MLF高耐受性优良乳酸菌的筛选与鉴定

Screening and Identification of Superior Tolerant Lactic Acid Bacteria for Grape Wine MLF

以活菌数为指标,以L450为对照菌株,考察了从自然发酵葡萄酒中分离的14株乳酸菌对酒精度、SO2浓度、p H值的耐受能力,筛选出高耐受性优良菌株。通过菌落形态、菌体形态、生理生化特征和16S r DNA分子测序进行优良菌株鉴定。结果表明:菌株G23与L42综合耐受能力最强且优于对照菌株L450,二者被鉴定为植物乳杆菌,可作为葡萄酒MLF高耐受性优良乳酸菌。

Fourteen strains tolerant to alcohol, sulfur dioxide and acid were isolated from natural fermented grape wine by using living bacteria number as major index and using strain L450as control strain. Excellent strains of wine malolactic fermentation （MLF） were obtained which with three superior tolerance characters for sulfur dioxide concentra- tion, alcohol concentration and acid concentration. We identified these excellent strains from the features in bacterial colony, micro-morphology, physiological and biochemical characteristics and 16S rDNA molecular sequencing. The results showed that G23 and L42 strains had the strongest comprehensive tolerance abilities and superior to L450, both of them were identified as Lactobacillus plantarum. They may be used as excellent MLF lactic acid bacteria for grape wine.