超高效液相色谱法检测6种人参皂苷含量

Determination of Six Ginsenosides by Ultrasonic Extraction-Ultra Performance Liquid Chromatography

采用超声提取药食同源植物（人参、西洋参、三七）中原人参三醇皂苷（Rg1、Re、Rf）和原人参二醇皂苷（Rb1、Rc、Rd）,建立了超高效液相色谱（UPLC）检测方法。以50%甲醇溶液为提取剂,料液比1∶80（g∶m L）,超声时间30 min。采用乙腈和0.05%磷酸水为流动相,梯度洗脱,检测波长为203 nm。该方法在质量浓度5~1 000μg/m L范围内线性良好,6种皂苷的最低检出限在22.5~51.0 mg/kg之间,平均回收率98.1%~105.5%。该方法准确、灵敏度高、重现性好、省时快捷,适合日常、大批量样品的检测。用此方法测定市售人参、西洋参及三七样品,结果表明3类样品中原人参二醇类皂苷含量高于三醇类皂苷,三七样品中Rg1的含量比人参和西洋参高,西洋参中Re含量高于人参和三七样品,而Rf仅在人参样品中检测到。

A method was developed for determination of portopanaxdiol（Rbl, Rc and Rd） and protopanaxtriol （Rgl, Re and Rf） type finsenosides in medicinal and edible plants （Ginseng, American Ginseng and Notoginseng） by ultrason- ic extraction-ultra performance liquid chromatography detection. The optimal extraction conditions were 50% methanol as the extraction solvent, material-to-liquid ratio 1：80 （g/mL） and extraction time 30min. The analyses were performed on UPLC using aeetontrile and 0.05% phosphoric acid solution gradient elution and with a detection wavelength of 203 nm. The experimental results showed a good linear relationship in the concentration range from 5 μg/mL to 1 000 μg/mL （R2 〉0.999）. The method detection limits of six ginsenosides were 22.5-51.0 mg/kg, the recoveries for them were 98.1%- 105.5%. The results showed that the method is rapid, convenient, accurate and applicable for the determination of gin- senosides in medicinal and edible plants. Based on this method, Ginseng, American Ginseng and Notoginseng samples were investigated. The data indicated that the content of portopanaxdiol type finsenosides were higher than protopanaxtriol. In three types of samples, the content of Rgl was the highest in Notoginseng, Re was the richest in American ginseng samples, Rf was only detected in Ginseng.