桃儿七地下茎内生真菌的分离和鉴定

Isolation and Identification of Endophytic Fungi Obtained from Underground Stems of Sinopodophyllum hexandrum

目的:探索甘肃兴隆山桃儿七内生真菌的多样性和丰度,为鬼臼毒素的获取方式提供新途径,保护濒危药用植物桃儿七资源。方法:采用组织块培养法从一株桃儿七地下茎中分离纯化内生真菌,利用分子鉴定和常规的形态学方法对重复出现的4株内生真菌进行分类鉴定。结果:本研究采用组织块内生菌分离方法分离纯化了20株内生真菌,鉴定的4株菌分别是赭曲霉Aspergillus ochraceus,Eucasphaeria capensis,布氏白僵菌Beauvetia brongniaritii和镰孢菌Fusarium sp.。结论:内生真菌的多样性和丰度与桃儿七的生境有关,甘肃兴隆山桃儿七内生真菌优势属为Aspergillus,Eucasphaeria,Beauvetia和Fusarium。

Objective： This study investigated the diversity and abundance of endophytic fungi of Sinopodophyllum hexandrum in Xinglong Mountain, Gansu Province, in order to provide a new way for obtaining podophyllotoxin from endophytic fungi, and protect endangered medicinal plants. Methods： The S. hexandrum endophytic fungi were isolated by tissue culture method, and identified with molecular and morphological data. Results： Twenty endophytic fungi were obtained from S. hexandrum underground stems, and four recurring endophytic fungi were identified as Aspergillus ochraceus, Eucasphaeria capensis, Beauvetia brongniaritii and Fusarium sp. Conclusion： Endophytic fungi diversity and abundance were related with host plants and environments, and the dominant endophytic genera in S. hexandrum in Xinglong Mountain are Aspergillus. Eucasphaeria. Beauvetia and Fusarium.