阿尔茨海默病大鼠模型中胰岛素样生长因子-1和Caspase-3蛋白表达的情况观察

Observation of Alzheimer's disease model rats of insulin like growth factor-1 and Caspase-3 protein expression

目的观察阿尔茨海默病大鼠模型中胰岛素样生长因子-1和caspase-3蛋白表达情况。方法 90只体重范围在(240±10g)的健康Wister大鼠,随机分为三组:观察组(β—淀粉蛋白建模大鼠)、对照组(生理盐水对照)和正常组,每组30只。用放射免疫法测定各组大鼠脑组织中的胰岛素样生长因子-1(IGF-1),western blot法检测各组Caspase-3蛋白的表达情况。结果观察组、对照组和正常组脑组织中IGF-1蛋白表达水平分别为[(0.87±0.14)μIU/ml、(2.59±0.27)μIU/ml、(2.67±0.24)μIU/ml],对照组、正常组的实验结果与观察组相比差异显著(P<0.05);观察组、对照组和正常组大鼠脑组织中caspase-3蛋白表达水平分别为[(69.72±11.39)μIU/ml,(31.22±6.98)μIU/ml,(32.53±7.26)μIU/ml],对照组和正常组的实验结果与观察组相比具有显著差异(P<0.05)。结论阿尔茨海默病模型大鼠脑组织中的IGF-1蛋白表达减少,Caspase-3蛋白表达增加;IGF-1蛋白和Caspase-3蛋白表达在阿尔茨海默病大鼠中呈负相关。

Objective To observe the change of insulin like growth factor-1and Caspase-3protein expression in Alzheimer＇s disease model rats.Methods 90 Wister rats,weight in the range of（240±10g）with normal cognitive function,were randomly divided into three groups,named as the normal group;the control group（normal saline control group（observation group）;Alzheimer＇s disease model rats）.Determination of brain tissues of insulin like growth factor-1with radioimmunoassay（IGF-1）,the expression of caspase-3protein by Western blotting.Results the IGF-in the brain tissue of model group rats 1protein expression is lower than the normal group;the observation group in brain tissues of rats with Caspase-3protein expression increased significantly.Comparison between groups with significant difference（P〈0.05）.Conclusion expression of IGF-1protein decreased in the model of brain tissue of Alzheimer＇s disease rats,expression of IGF-1protein was increased.IGF-1protein and IGF-1protein play roles in the development of Alzheimer＇s disease.