NO、ET-1在低压缺氧时脑血流量调节中的作用

Roles of nitric oxdide and endothelin-1 regulation of cerebral bood flow during acute hypoxia

目的本文主要探讨急性低压缺氧时NO和ET-1对脑血流量变化的调节作用。方法大鼠随机分为平原组和急性低压缺氧组,用电磁血流量计测定脑血流量,用Gess法和放免法分别测量血浆和脑NO2-、ET-1含量,用双波长分光光度法测量NOS活性。结果急性低压缺氧时脑血流量、脑含水量、血浆和脑组织NO2-、ET-1含量、NOS活性明显增高(P<0.05),脑ET-1/NO2-比值明显下降(P<0.05)。结论急性低压缺氧时,脑血流量增加,ET-1、NO均参与了脑血流量变化的调节,NO血管舒张作用大于ET-1收缩作用。

Objective The article was to investigate the roles of nitric oxide（NO）and enothelin-1（ET-1）in regulation of cerebral blood flow（CBF）during acute hypoxia.Methods Rats were divided into normoxic group（Control）and acute hypoxia（AH）,CBF were measured by electromagnetic blood flowmeter,the changes of plasm NO2-,ET-1contents,brain NO2-,ET-1contents and NOS activity were observed.Results Acute hypoxia caused an increase in CBF,plasm NO2-,ET-1contents,brain NO2- 、ET-1contents and NOS activity,the ratio of ET-1/NO2- in the brain were decreased,as compared with the control group.Conclusion NO and ET-1may be involved in the regulation of CBF in acute hypoxia,but the NO-dependent vasodilation was stronger than the ET-1-dependent vasoconstriction.