丹桂热淋丸薄层鉴别及蛇床子素的含量测定

Thin Layer Chromatography Differentiation and Content Determination of Osthole of Dangui Relin Pills

目的：建立丹桂热淋丸中主要药味的薄层鉴别及含量测定方法,以提高制剂质量标准。方法：采用TLC法对丹桂热淋丸中金银花、桂枝进行了定性鉴别研究;采用HPLC法测定制剂中蛇床子素的含量,选用：Kromasil ODS C18（4.6mm×250mm,5μm）色谱柱,甲醇-水（65∶35）为流动相,流速1.0m L·min^-1,检测波长：322nm。结果：TLC法可鉴别出金银花、桂枝的特征斑点,且专属性强,斑点清晰,阴性对照无干扰;蛇床子素在0.0382-0.2292μg范围内与峰面积呈良好的线性关系（r=0.9997）,平均回收率为100.09%,RSD=0.92%。结论：该方法准确可靠,可用于丹桂热淋丸的质量控制。

Object： To set up the method of the thin layer chromatography differentiation and content determination of the main ingredients in a prescription for advancing quality Standard. Methods： TLC was used to quantitative identified for Lonicerae Japonicae Flos、Cinnamomi Ramulus;To set up Content determination method of Osthole by HPLC,Kromasil ODS C18 chromatographic column（4.6mm×250mm,5μm）was used,mobile phase： methanol-water（65：35）as mobile phase,flow rate：1m L·min^-1,detection wavelength：322nm. Results： The characteristic spots on TLC plates was Distinguished without interference in the blank reference.The method has highly specific.The linear range of Osthole was 0.0382-0.2292μgμg（r=0.9997）.The average recover is 100.09%,RSD=0.92%. Conclusion： The method is accurate and reliable.It can use to control the quality of the Dangui Relin water pills.