丹参多酚酸盐联合聚维酮碘预防大鼠术后肠粘连

Salvianolate Combined with Povidone-iodine in Prevention of Intestinal Adhesion in Rats

目的观察丹参多酚酸盐联合聚维酮碘对大鼠术后肠粘连的影响,探讨其可能作用机制。方法选用SD雄性大鼠50只,随机分为对照组、模型组、丹参多酚酸盐组、聚维酮碘组(PVP-I组)及丹参多酚酸盐联合PVP-I组(联合组),每组10只。除对照组外,其余各组均制作肠粘连模型。对照组和模型组每天腹腔注射5%葡萄糖溶液;丹参多酚酸盐组、PVP-I组和联合组每天分别腹腔注射丹参多酚酸盐、PVP-I和二者联合,连续注射8天。大鼠于术后第7天开腹,肉眼观察各组大鼠术后粘连分级情况;酶联免疫吸附法(ELISA)检测各组大鼠血清和粘连组织白细胞介素-1β(IL-1β)、转化生长因子-β1(TGF-β1)水平。结果丹参多酚酸盐组及联合组肠粘连程度明显低于模型组(P<0.05);丹参多酚酸盐组、PVP-I组及联合组血清和粘连组织IL-1β表达水平均显著降低于模型组[(64.82±10.55)pg/mL、(70.93±16.06)pg/mL、(62.37±3.32)pg/mL vs(75.75±6.67)pg/mL,(178.90±17.28)pg/mL、(190.76±34.16)pg/mL、(168.40±25.14)pg/mL vs(220.78±18.31)pg/mL,P<0.05或P<0.01)。丹参多酚酸盐组、PVP-I组及联合组血清和粘连组织TGF-β1表达水平均显著降低于模型组[(36.77±5.25)pg/mL、(42.67±3.40)pg/mL、(21.90±1.15)pg/mL vs(54.93±4.75)pg/mL,(639.62±118.31)pg/mL、(645.32±56.95)pg/mL、(609.87±64.88)pg/mL vs(759.87±178.42)pg/mL,P<0.05或P<0.01)。结论丹参多酚酸盐、PVP-I及二者联合均能降低大鼠术后肠粘连程度,这可能与其降低血清和粘连组织中IL-1β和TGF-β1表达水平有关。

Objective To investigate the effects of salvianolate and povidone-iodine on postoperative intestinal adhesion in rats.Methods Fifty male SD rats were randomly divided into 5 groups：blank control group,model group,salvianolate group,povidone-iodine group and salvianolate combined with povidone-iodine group（combination group）,with 10 rats in each group.A intestinal adhesion modeling was established in rats,and then each group were given the corresponding drugs（intraperitoneal injection of 5%glucose solution,4mL/kg;salvianolate 10.9mg/kg：0.625%povidone-iodine,4mL/kg） for 8d.On the 7th day,rats underwent open surgery in abdomen to observe and grade intestinal adhesion by macroscopy.The level of interleukin-1β and transform growth factor-β1 in blood samples and adhesive tissue samples was determined by ELISA.Results Compared with model group,the grade of intestinal adhesion in salvianolate group and combination group was significantly lower（P〈0.05）;the level of IL-1βin blood samples and tissue samples in salvianolate group,povidone-iodine group and combination group were lower（blood：75.75±6.67pg/mL vs 64.82±10.55pg/mL,70.93±16.06pg/mL,and 62.37±3.32pg/mL;tissue：220.78±18.31pg/mL vs 178.90±17.28pg/mL,190.76±34.16pg/mL,168.40±25.14pg/mL;P〈0.05 or P〈0.0l）;the level of TGF-β1 in blood samples and tissue samples in salvianolate group,povidone-iodine group and combination group were lower（blood：54.93±4.75pg/mL vs 36.77±5.25pg/mL,42.67±3.40pg/mL,and 21.90±1.15pg/mL;tissue：759.87±178.42pg/mL vs 639.62±118.31pg/mL,645.32±56.95pg/mL,609.87±64.88pg/mL;P〈0.05 or P〈0.0l）.Conclusion Both of salvianolate and povidone-iodine and their combination can effectively prevent intestinal adhesion after abdominal operation in rats,which may be related to reduction of IL-1β and TGF-β1 in peripheral blood and tissues.