当归多糖对宫内缺氧新生大鼠海马区NSE、GFAP表达的影响

Effect of Angelica Polysaccharide on NSE and GFAP in hippocampus of intrauterine hypoxia neonatal rats

目的:探讨宫内缺氧对新生大鼠海马区NSE及GFAP表达的影响及当归多糖的干预。方法:孕14 d SD大鼠60只随机分为对照组(20)、缺氧组(20)和治疗组(20),缺氧组在孕14 d时缺氧,治疗组在缺氧前注入当归多糖,对照组不行缺氧干预。幼鼠出生时取脑组织进行固定、脱水、包埋、切片、免疫组织化学染色观察神经元特异性烯醇化酶(NSE)和胶质纤维酸性蛋白(GFAP)的积分光密度值(IOD)。结果:缺氧后新生鼠海马区NSE表达明显减少(P<0.05),GFAP表达明显增多(P<0.05);当归多糖组NSE表达明显高于缺氧组(P<0.05),GFAP表达明显低于缺氧组(P<0.05)。结论:8%O2宫内缺氧将导致新生鼠海马区神经元数量减少,神经胶质细胞数量增多;当归多糖能抑制宫内缺氧对新生鼠海马区神经的损伤。

Objective： To investigate NSE and GFAP in hippocampus of neonatal rats after intrauterine hypoxia and the intervention effect of Angelica Polysaccharide. Methods： 60 14 d-conceiving female SD rats were divided randomly into control group,hypoxia group and treatment group,20 rats in each group,hypoxia since the fourteenth day of pregnant,the rats of angelica polysaccharide group were injected with angelica polysaccharide by stomach tube before hypoxia. Neonatal rats＇ brain was taken at birth immediately. Then fixed,dehydrated,embedded,sliced,and did immunohistochemical staining. Results： The IOD of NSE in hippocampus of neonatal rats after hypoxia was significantly decreased than that of Control group（ P〈0. 05）,the IOD of GFAP was increased significantly than that of Control group（ P〈0. 05）. The IOD of NSE in treatment group was significantly increased than that of hypoxia group（ P〈0. 05）,the IOD of GFAP in treatment group was significantly decreased that of hypoxia group（ P〈0. 05）. Conclusion： 8% oxygen intrauterine hypoxia,the number of neurons in hippocampus of newborn rats decrease,and increase in the number of glial cells. Angelica sinensis polysaccharide may inhibit the intrauterine hypoxia injury of hippocampus neural cells of newborn rats.