开设“流式细胞术”实验课 提高研究生实践能力

Setting Flow Cytometry Experiment Lesson to raise Graduates' Practice Ability

流式细胞术是一门新兴的实验科学技术,广泛应用于基础研究与临床检测等领域。根据科研的需要,面向兽医学相关专业的研究生开设了流式细胞术的实验课,帮助学生深入了解了流式细胞仪的工作原理、操作方法及应用。课程中设置了"PLA2G7-EGFP质粒转染ECA-109细胞与PK-15细胞后EGFP蛋白的表达"和"小鼠外周血单个核细胞(PBMC)的分离及B细胞的检测"两个教学范例。在课程中让学生亲自参与样本的制备,包括细胞的培养与转染、小鼠外周血的采集、单细胞悬液的制备、细胞染色、样本过滤、流式样本的上机操作、数据的获取与分析等,培养了学生的动手能力及独立操作机器的能力。并与研究生以后的科研实验相结合,提高了学生独立思考、分析问题、解决问题的能力,帮助研究生在未来的实验研究中科学规划和使用流式细胞仪。

Flow cytometry is an emerging experimental science and technology, widely used in basic research and clinical testing and other scopes. According to research related to veterinary science-oriented graduate students, flow cytometry laboratory sessions were opened to help students understand the working principle of the flow cytometer, methods of operation and application. Two teaching courses ＂ PLA2G7-EGFP plasmid expressing EGFP protein ECA-109 after PK-15 cells＂ and ＂ detecting peripheral blood mononuclear cells （PBMC） and B cells isolated＂ were set up. In the curriculum, students were involved in the preparation of samples, including cell culture and transfection, mouse peripheral blood collection, preparation of single cell suspensions, cell staining. The sample was filtered stream samples machine operation, acquired data and analysis. It trained the students＂ practical ability and the ability to operate independently of the machine. Base on these lessons, experimental research and graduate ability later was combined to improve the students in thinking independently, and enhance their analyzing problems, problem-solving skills. It also helps students to use scientific planning and study of flow cytometry experiments in the future.