木荷优良家系的组织培养研究

Tissue Culture and Micropropagation of Superior Family of Schima superba

以新选育的木荷(Schima superba)优良家系为试材,开展组织培养。结果表明,木荷适宜的初代培养基为GD+6-BA 1.50 mg·L-1+NAA 0.50 mg·L-1,继代培养基为GD+6-BA 1.00 mg·L-1+IAA0.30 mg·L-1,生根培养基为1/4 MS+IBA 1.00 mg·L-1+IAA 0.40 mg·L-1。芽苗增殖倍数为3.1倍,生根率达96.67%。探索出适宜的驯化、移栽和后期管理技术,使优良家系生根苗的移栽存活率达到92.2%。

A newly-selected superior family of Sehima superba was used for tissue culture and micropropagation. The explants of stem segments displayed the optimum initial growth on the primary media GD＋6-BA 1.50 mg · L^-1＋NAA 0.50 mg· L^-l, the highest proliferation rate of 3.1 times on subculture GD＋6-BA 1.00 mg·L^-1＋IAA 0.30 mg·L^-l, and 96.67% rooting rate on medium 1/4 MS ＋ IBA 1.00 mg·L^-1 ＋IAA0.40 mg·L^-1. Suitable techniques and procedures were identified for acclimatization, transplantation and later-period management of tissue-cultured plantlets. The survival rate of the superior family was 92.2%.