基于HRP直接标记的流感病毒H1N1电化学免疫传感器

A Novel Electrochemical Immunosensor for Influenza A( H1N1)Virus Recognition Based on HRP Direct Labelling

构建了一种新型、灵敏、便捷式流感病毒免疫传感器,通过在金电极表面键合抗-HA单克隆抗体,选择性捕获目标H1N1流感病毒。该方法基于吸附在病毒表面的辣根过氧化物酶（HRP）在亚甲基蓝（MB）溶液中可有效催化还原H2O2,利用方波伏安法（SWV）考察了还原峰电流的变化,从而实现了对抗原病毒的特异性识别。实验表明所构建的免疫传感器对H2O2-MB体系表现出快速的电流响应以及良好的稳定性,对流感病毒H1N1检测的动态响应范围为0.01～2.0μg/m L,检出限（S/N=3）为5.0 ng/m L。结果证明HRP可作为一种简单快速的探针用于流感病毒的实时监测。

An immunosensor for the sensitive and simple recognition of low concentrations of influenza viruses was constructed. Gold electrode was coated with HI subtype -specific monoclonal antibodies to selectively capture the target virus. Horseradish peroxidase（HRP） as a catalyst to reduce hydrogen peroxide （H2O2 ） in the methylene blue （MB） solution was immobilized on the biosensor. The change of reduction peak current for specific recognition of antigen virus was monitored by square wave volta- mmetry （SWV）. The biosensor exhibited a fast amperometric response and a good stability to H2 02 - MB system. The dynamic range for antigen recognition was in the range of 0.01 -2. 0 μg/mL with a detection limit （ S/N = 3 ） of 5.0 ng/mL. The results indicated that HRP had a good sensitivity and a simple operation for the detection of influenza virus.